多沙唑嗪对映体对老年小鼠前列腺增生的影响  

Effects of doxazosin enantiomers on the volume and cell proliferation of the prostate in aged mice

作　　者：卢海刚[1] 刘丽芳[1] 边虹铮[1] 段丽华[1] 王乐[1] 

机构地区：[1]河北化工医药职业技术学院,河北石家庄050026

出　　处：《中国药理学通报》2013年第6期828-831,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30940088);国家科技部"重大新药创制"科技重大专项资助项目(No 2009ZX09502-022);河北省自然科学基金资助项目(No C2009001070);河北省教育厅科研资助项目(No 208108);河北化工医药职业技术学院科研资助项目(No YZ1007)

摘　　要：目的观察多沙唑嗪[(±)Dox]及其对映体[(-)Dox和(+)Dox]对老年小鼠前列腺体积、湿重、细胞增殖周期与凋亡百分率的影响。方法取13月龄♂小鼠,灌胃给予(±)Dox及其对映体(-)Dox和(+)Dox连续12周,采用流式细胞技术观察药物对小鼠前列腺增生的影响。结果与青年对照组相比,老年组小鼠前列腺湿重明显增加(P<0.05);与青年对照组相比,老年组小鼠前列腺体积指数差异无显著性,而(-)Dox 6.0 mg.kg-1组、(+)Dox 6.0 mg.kg-1组和(+)Dox 0.6 mg.kg-1组以及(±)Dox 6.0 mg.kg-1组小鼠前列腺体积指数明显减小(P<0.05)。与青年对照组相比,老年组小鼠前叶前列腺G0/G1期细胞周期百分率显著降低(P<0.01),G2/M期细胞周期百分率和增殖指数均显著增高(P<0.01);老年组小鼠前列腺细胞凋亡率无明显改变。与老年组相比,(±)Dox及其对映体(-)Dox和(+)Dox对老年小鼠前列腺细胞的增殖指数和细胞周期无明显影响。结论长期(12周)灌胃给予(±)Dox及其对映体(-)Dox和(+)Dox,可轻度降低老年小鼠前列腺细胞体积指数。Aim To study the effects of long-term ad- ministration of （-） doxazosin [（-） Dox], （＋） doxazosin [ （ ＋ ） Dox ] and （± ） doxazosin [ （ ±） Oox ] on the volume and cell proliferation of the prostate in aged mice. Methods （-）Dox, （ ＋）Doxand （±） Dox were administered intragastrically to the male mice at the age of 13 months for 12 weeks to analyze the effects of the three agents on the volume and wet weight of the prostate, and the effects on the cell proliferation and apoptosis of the anterior lobe prostatic cells in aged mice. Results In comparison with young mice, the wet weight of the prostate in aged mice significantly in- creased （P 〈 0. 05）. In comparison with young mice, the volume index of the prostate in aged mice did not change significantly （P 〉 0. 05 ）, but the volume index of the prostate in aged mice treated with （-） Dox （6.0 mg. kg-1）, （ ＋）Dox （6.0rag. kg-1）, （＋）Dox （0.6 mg . kg-1） or （ ±）Dox （6.0 mg. kg-1） was significantly decreased （P 〈 0. 05 ）. In comparison with young mice, the total G0/Gl-phase cells of the anterior lobe of the prostate were significantly de- creased （P 〈 0. 01 ）, and the total G2/M-phase cells of the anterior lobe of the prostate and the proliferation in- dex of the prostatic cells were significantly increased in aged mice （P 〈 0.01 ）. In comparison with aged mice, the proliferation index and cell cycle distribution of the anterior prostatic lobe in aged mice treated by （ - ） Dox, （ ＋ ） Dox and （± ） Dox did not change sig- nificantly （P 〉 0. 05）. Conclusion These results in- dicate that doxazosin and its enantiomers slightly de- crease the volume index of the prostate in aged mice by long-term intragastrical administration.

关 键 词：多沙唑嗪 对映体 细胞增殖 凋亡 前列腺 老年小鼠 

分 类 号：R-332[医药卫生] R322.64