低温纤维素降解复合菌系的选育及性能分析  

Breeding and Properties Analysis of Low-Temperature Cellulose-Degrading Strains

作　　者：盛铭浩[1] 徐凤花[1] 代欢[1] 张晴[1] 

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030

出　　处：《湖北农业科学》2013年第8期1814-1816,1828,共4页Hubei Agricultural Sciences

基　　金：国家"十二五"科技支撑计划项目(2012BAD14B06)

摘　　要：以腐木和堆肥样品为材料,通过限制性继代培养和混合培养,筛选出一组高效稳定的低温纤维素降解复合菌系Y。对复合菌系Y不同生长阶段pH、酶活、失重率及产物之间的相互关系进行了研究。结果表明,0～6 d为复合菌系Y的对数生长期,4 d内滤纸条完全崩解,在20℃下静置培养10 d,水稻秸秆的降解率达到59%,酶活为103.5 U/mL,主要代谢产物为乙酸,并有少量的丁酸和丙酸,含量分别为1.70、0.21、0.12 g/L,其中乙酸占总挥发酸含量的85%。复合菌系Y适用于以秸秆为原料的低温产沼气发酵。Using rotten wood and composting samples as materials,through restrictive subculture and mixed culture,a group of composite strain Y with efficient and stable cellulose-degrading ability were screened.The relationship among pH,enzyme activity,weightlessness rate and products in different growth stages of strains Y were sludied.The results showed that the 0 to 6 days were the logarithmic growth phase of strain Y;filter strips completely collapsed in four days.Static culture at 20 ℃ for 10 d,rice straw degradation rate reached to 59% whele enzyme activity was 103.5 U / mL.The main metabolic products were acetic acid and a small amount of butyric acid and propionic acid,their content was 1.70,0.21,0.12 g / L,respectively.Acetic acid accounted for 85% of the total volatile acid content.Composite strains Y was suitable for being applied to lowtemperature methane fermentation with straw as raw materials.

关 键 词：低温纤维素降解复合菌系 选育 水稻秸秆 代谢产物 

分 类 号：X172[环境科学与工程—环境科学]