不同精粗比底物下不同添加剂对绵羊瘤胃体外发酵的影响  

Effect of different additives on in vitro rumen fermentation differing in forage-to-concentrate ratios

作　　者：杨平平[1] 甄玉国[1] 王晓磊[1] 赵巍[1] 于中英[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《饲料工业》2013年第9期25-29,共5页Feed Industry

摘　　要：试验旨在比较研究不同精粗比底物下酵母培养物、米曲霉提取物和植物精油对绵羊瘤胃体外发酵的影响。采用两因素交叉分组试验设计,因素一有4个水平,分别为对照组、添加酵母培养物、米曲霉提取物、植物精油;因素二有3个水平,分别为精粗比3:7、5:5、7:3;培养24 h进行采样并测定培养液pH值、NH3-N、VFA浓度。结果表明:①从整体趋势看,随着精粗比的增加,培养液VFA浓度逐渐增加,pH值、NH3-N浓度逐渐降低。②YC组在各精粗比底物下与对照组相比,极显著提高培养液VFA浓度,极显著降低pH值(P<0.01),对NH3-N浓度的影响仅在精粗比3:7和5:5达到显著水平(P<0.05)。③AO组在精粗比5:5底物下,与对照组相比,极显著增加乙酸、TVFA浓度(P<0.01);在精粗比7:3底物下,与对照组相比,显著增加乙酸(P<0.05),极显著增加TVFA浓度(P<0.01)。④EO组在精粗比7:3底物下,与对照组相比,极显著增加乙酸、丙酸和TVFA浓度(P<0.01),显著增加丁酸浓度(P<0.05)。This study was designed to evaluate effects of different additives on in vitro remen fermentation differing in forage-to-concentrate ratios. It was two factors cross group design, one factor had four levels of control, YC, AO and EO, the other factor had 3 different levels of forage to concentrate which were 3 ： 7, 5 ： 5, 7 ： 3; After 24 h of incubation, fermentation fluid was collected and analyzed for pH, NH3-N and VFA. The results showed as follows： （1） With concentrate to forage ratios inereased, VFA concentration of the fermentation fluid were higher, pH and NH：N concentration were lower（P〉0.05）. （2） YC increased VFA concentration and decreased pH and NH3-N concentration in different concentrate to forage ratio diets compared with control（P〈0.01）; and decreased NH：N concentration in C ： F 3 ： 7 and 5 ： 5 diets（P〈 0.05）.（3）AO increased acetate and TVFA （P〈0.01） in a C ： F 5 ： 5 ratio diet; in a C ： F 7 ： 3 ratio diet, AO increased aeetate（P〈O.05）and TVFA（P〈0.01 ）. （4） EO increased acetate, propiohate, TVFA concentration（P〈0.01）, and butyric（P〈0.05）in a C ： F 7 ： 3 ratio diet compared with control.

关 键 词：精粗比 酵母培养物 米曲霉提取物 植物精油 体外发酵 

分 类 号：S823[农业科学—畜牧学]