一氧化氮调节癌细胞中钙离子动力学  

Nitric Oxide Modulating Intracellular Calcium Dynamics of Cancer Cells

作　　者：陈江旭[1] 黄义梅[1] 杨洪钦[1] 郑莉琴[1] 王瑜华[1] 谢树森[1] 

机构地区：[1]福建师范大学激光与光电子技术研究所,医学光电科学与技术教育部重点实验室,福建省光子技术重点实验室,福建福州350007

出　　处：《福建师范大学学报（自然科学版）》2013年第3期59-62,共4页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(60978071);福建省自然科学基金资助项目(2010J01322)

摘　　要：一氧化氮(NO)既能促进又能抑制癌细胞的增殖与转移,但是NO在癌细胞增殖与转移过程中所起作用的分子机理尚未明确.采用微孔板荧光检测技术观察NO对癌细胞钙离子浓度的调节作用,实验结果显示,外源性NO能使细胞内钙离子荧光强度显著增强,且增强程度与NO质量分数呈正相关;此外,NO引起的细胞内钙离子浓度增大主要是源于细胞内钙库的钙释放.研究结果有助于在细胞分子层面认识NO在癌细胞增殖与转移中的作用机制.NO has the ability to promote or inhibit cancer cell proliferation and metastasis, however, the molecular mechanism of NO involving in this process is still unclear. It investigated the effects of NO on the intracellular calcium dynamics of HeLa cells by microplate fluorescence reader, results showed that exogenous NO increased intracellular Ca2 ~ fluorescence intensity signifi- cantly, which was proportional to NO concentrations. Furthermore, the increase of the calcium in- duced by exogenous NO was mainly due to calcium releasing from intracellular calcium stores. These results would be helpful to recognize the mechanism of NO modulating cancer cell growth and metas- tasis at cellular and molecular level.

关 键 词：一氧化氮 钙离子 HELA细胞 微孔板荧光分析仪 生物光子学 

分 类 号：TN247[电子电信—物理电子学] R730.23[医药卫生—肿瘤]