狂犬病病毒RT-LAMP检测方法的建立  

Establishment and Application of RT-LAMP Method for Detecting Rabies Virus

作　　者：何媛[1,2] 罗宝正[2] 邵建宏[2] 薄清如[2] 徐海聂[2] 林瑞庆[1] 沙才华[2] 廖秀云[2] 陈步雲 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]珠海出入境检验检疫局,广东珠海519015 [3]珠海市香洲区宠物行业协会,广东珠海519015

出　　处：《动物医学进展》2013年第5期1-5,共5页Progress In Veterinary Medicine

基　　金：珠海出入境检验检疫局科技计划项目(ZH2011-3)

摘　　要：本研究利用逆转录环介导等温核酸扩增技术(RT-LAMP),以狂犬病病毒的核蛋白基因保守区段设计LAMP引物,建立了狂犬病病毒的RT-LAMP快速检测方法,并对建立的方法进行了特异性、灵敏度和稳定性试验。结果显示,该方法检测结果可直接用肉眼判断,重复性好,稳定可靠,可将狂犬病病毒与犬瘟热病毒(CDV)、犬副流感病毒(PIV)、犬腺病毒(CAV)、犬细小病毒(CPV)进行鉴别,对86份犬唾液样品和3种商品化狂犬病疫苗进行检测表明,建立的方法适用于样品中狂犬病病毒的临床检测。A reverse transcription loop-mediated isothermal amplification （RT-LAMP） method for detection of rabies virus was developed using a set of six primers designed according to conservative section of the ra- bies virus gene to detect specificity, sensitivity and stability. The results of RT-LAMP were seen by na- ked-eye, with ideal specificity, and could distinguish rabies virus with CDV, PIV, CAV and CPV, and the sensitivity was slightly higher than that of Real-time RT-PCR. The whole detection of RT-LAMP need not open the lid. 86 saliva samples and 3 kinds of commercial vaccines were detected, and showed that this method is suitable for the clinical detection of rabies virus, as well as the live virus in the vaccine or inactivated virus.

关 键 词：狂犬病病毒 逆转录-环介导等温核酸扩增技术 检测 

分 类 号：S852.65[农业科学—基础兽医学]