响应面法优化加拿大原产地西洋参皂苷的提取工艺研究  

Optimization of Extraction Process of Ginsenoside from Panax quinquefolius L. Collected from Canada by Response Surface Methodology

作　　者：许云章[1] 任烨[1] 王静霞[1] 孙美[1] 赵小燕[1] 刘圆[1] 

机构地区：[1]西南民族大学民族医药研究院,四川成都610041

出　　处：《现代食品科技》2013年第5期1040-1044,1035,共6页Modern Food Science and Technology

基　　金：国家科技部"重大新药创制"(2011ZX09307-002-01);国家科技支撑计划(2012BAI27B07);国家自然基金(81173653);四川省杰出青年学术技术带头人后续计划(2011JQ0051)

摘　　要：优化加拿大原产地西洋参皂苷的最佳提取工艺。采用HPLC-ELSD法,在单因素试验基础上,利用响应面法,以人参皂苷Rb1为响应值,建立数学模型,对提取温度、时间、乙醇浓度和料液比进行优化,确定最佳提取工艺。结果表明:提取温度为90℃、乙醇浓度77.4%、提取时间1.88 h、料液比1:25、提取2次的人参皂苷Rb1提取率可达到1.40%,与预测值1.41%相差不大。The Response Surface Methodology 0LSM） was used to optimize the extraction process of ginsenoside from P quinquefolius L. collected from Canada. Based on one-factor-at-a-time experiments, extract yield of the ginsenoside-Rbl was used as response for establishing a quadratic regression model. Temperature and time of extraction, ethanol concentration and liquid-to-solid ratio were selected as the factors for RSM design. The results showed that the best extraction conditions were as follows： extraction temperature 90 ~C, extraction time 1.88 h, ethanol concenlration 77.4%, solid-liquid ratio 1：25, and extracted times twice, under which the predicted yield of ginsenoside-Rbl with the optimized procedure was up to 1.40%and practical yield was 1.41%.

关 键 词：加拿大 西洋参 人参皂苷RB1 响应面法 提取工艺优化 

分 类 号：TQ461[化学工程—制药化工]