华蟾素对喉癌细胞凋亡的基础研究  

Basic Study of cell apoptosis for cinobufacini on human laryngeal carcinomacell

作　　者：韩仲明[1] 苏红星[2] 黄晋生[3] 张海蓉[3] 郑集义 

机构地区：[1]海军总医院耳鼻咽喉科,北京100037 [2]首都医科大学组胚教研室 [3]海军总医院中心实验室 [4]海军总医院病理实验室

出　　处：《中国中西医结合耳鼻咽喉科杂志》2000年第3期111-114,共4页Chinese Journal of Otorhinolaryngology in Integrative Medicine

基　　金：全军医学科研课题!96MD03

摘　　要：目的探讨中药华蟾素对体外培养人喉癌Hep－2细胞生长作用及癌基因变化。方法应用光镜，电镜；MTT检测技术；SP免疫络化技术及流式细胞分析仪，对药物作用后Hep－2细胞的数量，形态，抑癌基因P53，癌基因cmyc，Bcl-2蛋白的表达以及细胞周期DNA含量的改变进行研究。结果发现华蟾素对Hep－2细胞作用在低浓度短时间时促进细胞分裂、增殖和诱导细胞分化；高浓度长时间则抑制细胞生长，促进细胞凋亡。华蟾亲对Hep－2细胞癌基因影响表现为P53蛋白表达略增加，c－myc，Bcl-2蛋白表达降低。华赠素对Hep-2细胞周期DNA含量的影响表现为阻断DNA从G1向S期转化。结论华蟾素对喉癌细胞的生长及癌基因有特异性作用，可作为临床喉癌综合治疗的用药之一。Objective To investigate the effects of cinobufacini on oncogenes in human la-ryngeal carcinoma cell line Hep- 2. Method In our experiment, the effect of cinobufacini on theproliferation of Hep-2 was observed by using the following methods: the morphologic changesof the cells were observed with optical and transmission electron microscopy, the cytotoxicity ofcinobufacini to these cell lines were measured by MTT assay, the expresser gene was determinedwith SP immunohistochem1stry, flow cytometry and image analysis. Result The results showedthat when Hep-2 cells were treated with cinobufacini for 24 hours, the cell proliferation was in-duced and cell became well -differentiated. When Hep -2 cells treated for 120 hours, the celldeath occurs by apoptosis. The expression of the oncogenes C-myc and Bcl-2 protein were re-markably decreased whereas that of the tumor suppresser gene P53 protein seems to increas.DNA content in the cycle progression of Hep - 2 cell was assessed by flow cytometry. lt wasfound that after Hep - 2 treated with cinobufacini 24 hours DNA synthesis was suppressed withsignificant GI block. Conclusion The antitumor effect and induce for the differentiation ofclnobufacini might be by Ineans of inhibiting the expression of oncogenes and improv1ng the ex-pression of carcinoma suppresser gene.[

关 键 词：华蟾素 HEP-2 癌基因 喉癌 免疫组化 流式细胞 

分 类 号：R739.65[医药卫生—肿瘤]