诱导子对藏红花悬浮培养细胞生产藏红花色素的影响  

Effects of elicitors on saffron pigment production in cell suspension cultures of Crocus sativus L.

作　　者：孙镇[1] 袁丽红[1] 吴频梅[1] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009

出　　处：《生物加工过程》2013年第3期18-23,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)(2009CB724700)

摘　　要：考察壳聚糖(chitosan)、壳寡糖(chitosan oligosaccharides,COS)、茉莉酸甲酯(methyl jasmonate,MJ)、水杨酸(salicylic acid,SA)和Cu2+等诱导子对藏红花悬浮培养细胞生长和藏红花色素合成的影响。结果表明:在实验考察浓度范围内,壳寡糖(1～500 mg/L)和较低浓度壳聚糖(≤10 mg/L)、MJ(≤10μmol/L)、SA(≤10μmol/L)和Cu2+(≤1μmol/L)对细胞生长无显著影响;较高浓度壳聚糖(≥100 mg/L)、MJ(≥100μmol/L)、SA(≥100μmol/L)和Cu2+(≥10μmol/L)显著抑制细胞生长。5种诱导子对藏红花色素合成的诱导效果不同,并且与诱导子作用浓度和添加时间有关。MJ诱导效果最好,在细胞培养第0天添加终浓度100μmol/L MJ,藏红花色素含量(以1克干细胞计)达到28.57 mg,比对照提高177.9%。其次是Cu2+,在细胞培养第4天添加终浓度500μmol/L Cu2+,色素含量达到19.82 mg,比对照提高108.2%。再次是壳聚糖和壳寡糖,在细胞培养第14天分别添加终质量浓度100mg/L壳聚糖和壳寡糖,色素含量分别达到18.33和17.39 mg,比对照提高69.1%和69.0%。最后是SA,在细胞培养第14天添加终浓度10μmol/L SA,色素含量达到14.65 mg,比对照提高45.4%。The effects of chitosan, chitosan oligosaccharides （ COS ）, methyl jasmonate （ MJ ）, salicylic acid （SA） ,and copper ions（ Cu2＋） on cell growth and saffron pigment production in cell suspension cultures of C. sativus were investigated. The results showed that COS（ 1 -500 mg/L）, chitosan（ ≤ 10 mg/L）, MJ （ ≤10 μmol/L） ,SA（ ≤ 10 μmol/L） ,and Cu2＋ （ ≤1 μ mol/L） had no significant effects on saffron cell growth, whereas chitosan （ ≥ 100 mg/L）, MJ （ ≥ 100 μmol/L）, SA （ ≥100 μmol/L）, and Cu2＋ （≥ 10 μ mol/L） at high concentrations obviously inhibited the cell growth. The effects of elicitors were significant on saffron pigment biosynthesis,and had relations with the concentrations of elicitors and the timing of elicitation. MJ was the best elicitor,the content of saffron pigment reached 28. 57 mg/g DW by adding 100 μmol/L MJ on day 0,and it was higher by 177.9% than the control. Cu2＋ was the second,the content of saffron pigment reached 19. 82 mg/g DW by adding 500 μmoL/L Cu2＋ on day 4, and it was higher by 108. 2% than the control. Chitosan and COS were the 17.39 mg/gDW by adding 100 mg/L third, the contents of saffron pigment reached 18.33 mg/gDW and chitosan and oligochitosan on day 14,and it was higher by 69. 1% and69. 0% than the control, respectively. The last one is SA, the content of saffron pigment reached 14. 65 mg/gDW by adding 10 μmol/L SA on day 14 and it was higher by 45.4% than the control.

关 键 词：细胞悬浮培养 藏红花 诱导子 藏红花色素 

分 类 号：Q943.1[生物学—植物学]