卵巢肿瘤细胞凋亡与细胞增殖的关系  

Correlation of apoptosis and cell cycle control in ovarian tumors

作　　者：李利[1] 苏杏满[1] 程建新[1] 左连富[1] 宋俊芬[1] 杨静 

机构地区：[1]河北医科大学第四医院妇产科,石家庄市050011

出　　处：《现代中西医结合杂志》2000年第14期1307-1310,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的 :评估卵巢肿瘤细胞凋亡与增殖状况及两者的关系。方法 :运用流式细胞术检测良交恶三组卵巢肿瘤中bcl-2、p16、Rb,Cyclin D1蛋白及 TGF-β1 因子的表达和 DNA含量 (恶性组 3 3例、交界组 12例、良性组 5例 )。各指标间的相关性通过单因素直线相关分析和多因素直线相关分析来评估。结果 :bcl-2和 AP%间 ,TGF-β1 和 PI间俱呈负相关 ( r=-0 .2 8,P<0 .0 5 ,r=-0 .2 5 ,P<0 .0 5 )。多因素相关分析发现 p16的 F I值和 PI值呈负相关 ( Yi=-2 0 .3 2 ,P=0 .0 81)。恶性组和交界组卵巢肿瘤的 AP%值与良性肿瘤比较发生了相对减少。多因素分析证实 A P%值与 DI值呈负相关 ( Yi=-9.96,P=0 .0 3 7)。恶性组的细胞累积率高于良性组 ( P<0 .0 1)和交界组 ( P<0 .0 5 )。bcl-2和 p16呈负相关 ( r=-0 .2 6,P<0 .0 5 )。结论 :bc L -2能够抑制凋亡 ,它的增加有助于细胞恶性转变。 TGF-β1 能够抑制增殖 ,细胞对 TGF -β1 反应性的降低有助于恶变。 p16、 Rb的缺失和 Cyciln D1的增加有助于卵巢癌的发生和发展。恶性肿瘤中 AP%值的相对下降对于肿瘤恶变具有重要意义。 bcl-2和 p16间的负相关提示凋亡和增殖间具有相互作用。正由于凋亡相关蛋白和细胞周期调节因子间的相互作用 ,使恶性肿瘤保持较高的细胞累积率。Objective: The study assessed how about apoptosis and cell cycle control in ovarian tumors and correlation of both. Methods: Flow cytometric analysis was used to measure bcl 2, p16, Rb, cyclin Dl proteins, TGF β 1 (Transforming growth factor β 1) cytokine expression and DNA content in three pathological type tumors (33 malignant, 12 boderline and 15 benign). The correlation between parameters was evaluated by simple least squares linear regression and multiple linear regression.Results: A negative correlation was found between TGF β 1 and PI (r=-0.25, P<0.05). A linear negative correlation between median FI of p16 and p1 was found by multiple linear regression (Yi=-20.316599, P=0.0081). Compared with benign tumor, Ap% of malignant and borderline ovarian tumors lowered. There was a negative correlation between AP% and DI found by multiple linear regression(Yi=-9.964683,P=0.0371). Cell accumulation rate of malignant tumors was higher than benign tumors(P<0.01) and borderlines tumors (P<0.05). A negative correlation between bcl 2 and p16 was found (r=-0.26, P<0.05).Conclusions: bcl 2 can inhibit apoptosis and the increase induce transition to malignancy. TGF β 1 can inhibit proliferation and the reduce of cell responsiveness to TGF β 1 contribute to transition to malignancy. The loss of p16, Rb and increase of Cyclin D1 contribute to occurrence and development of ovarian cancer. The comparatively reduce of AP% in malignant tumors is important to tumorigenesis. The negative correlation between bcl 2 and p16 indicate that apoptosis and proliferation have reciprocal activation. It is because the reciprocal activation of apoptotic proteins and cell cycle control factors that maintain a higher cell accumulation rate in malignant tumors.

关 键 词：卵巢肿瘤 P16 RB CYCLIND1 TGF-Β1 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]