单个胎儿有核红细胞的DNA分析  

Genetic analysis of single fetal nucleated red blood cell from maternal blood

作　　者：邹丽[1] 朱剑文[1] 张会军 李日文 李敏 

机构地区：[1]同济医科大学附属协和医院妇产科,武汉430022

出　　处：《临床血液学杂志》2000年第4期175-176,共2页Journal of Clinical Hematology

基　　金：国家教委留学回国人员启动基金!资助项目 (No.96 6 44 );卫生部回国人员启动基金!资助项目 (No. 96 0 2 412 )

摘　　要：目的 :获取孕妇外周血中的极少量胎儿有核红细胞 (NRBC) ,并进行单个 NRBC的基因分析 ,探讨其在产前基因诊断中的价值。方法 :将孕妇外周血进行不连续密度梯度离心 ,将分离后的细胞进行制片 ,光学显微镜下识别 NRBC,然后用显微操作仪获取单个 NRBC进行引物延伸预扩增 (PEP)及 Y染色体特异性 DYZ1 基因的聚合酶链反应 (PCR) ,以确定其来源于胎儿。结果 :单个 NRBC的 PEP- PCR法检测结果 ,6 0 %检出 DYZ1 的特异性条带 ,而单用 PCR法则均未检出特异性 DYZ1 条带。结论 :用母血中单个胎儿 NRBC进行产前基因诊断是可行的。Objective:To study the feasibility of usintg single fetal nucleated red blood cell(NRBC) for prenatal genetic analysis.Method:Fetal cells were isolated from maternal blood with discontinuous density gradient centrifugation.Single NRBC was found and retrieved using a micromanipulator under a microscope.To determine whether the origin of the NRBC was maternal of fetal,the single NRBC was analysed by primer extension preamplification(PEP)and polymerase chain reaction(PCR)to determine the presence of a Y chromosome specific repeat sequence.Result:We have determined the presence of DYZ 1 specific repeat sequence by single NRBC. The male pregnancy was correctly predicted in 60%.Conclusion:Using single fetal NRBC for prenatal genetic analysis is feasible.

关 键 词：妥妇外周血 单个胎儿有核红细胞 产前诊断 

分 类 号：R714.5[医药卫生—妇产科学]