实验性再狭窄动物模型研究  

A study on the animal model of experimental restenosis

作　　者：李自成[1] 常青[2] 

机构地区：[1]暨南大学第一附属医院心内科,广州510630 [2]暨南大学医学院组织胚胎学教研室

出　　处：《解剖学研究》2000年第1期22-24,共3页Anatomy Research

基　　金：国务院侨办重点基金;广东省中医药管理局科研基金资助课题

摘　　要：目的　为弄清经皮冠状动脉腔内形成术后动脉再狭窄的发生机制 ,本实验研究建立再狭窄动物实验模型。方法　采用右髂动脉球囊内皮剥脱术加高胆固醇喂饲家兔。结果　经血管造影检查 ,血管成形术前右髂动脉平均狭窄程度在6 0 %以上 ,血管成形术后 5周右髂动脉平均再狭窄程度在 5 0 %以上 ;病理切片和组织生化显示 ,内膜明显增厚 ,可见大量泡沫细胞 ,脂质沉积显著增加 ;透射电镜和免疫组织化学鉴定显示 ,增生内膜主要是平滑肌细胞增殖。结论　采用本方法成功地建立了再狭窄动物实验模型。Objective To establish the animal model of restenosis.Mehtods The model of atherosclerotic stenosis was reproduced by balloon endothelial denodation of right iliac artery in cholesterol fed rabbit.Results With right iliac artery angiography,the degree of stenosis was averagely more than 60% before angiography,and the degree of restenosis was averagely more than 50% in 5 weeks after angiopasty.Pathological sections were showed with marked thicking of intima comprising of abundent foam cells and the accumulation of cholesterol was significantly increased in the right iliac artery.The results of identification by transmission electron microscopy and immunohistochemistry showed that the hyperplastic intima was mainly composed of the proliferated smooth muscle cells.Conclusion The results suggested that the animal model of restenosis be successfully established.

关 键 词：内皮剥脱术 血管成形术 再狭窄 动物模型 

分 类 号：R-332[医药卫生]