Λ-WH0402对人脐静脉内皮细胞增殖、迁移的抑制作用  

Inhibition of Λ-WH0402 on the proliferation and migration of human umbilical vein endothelial cells

作　　者：袁健[1] 陈东波[1] 吕小迅[1] 

机构地区：[1]广东药学院药用生物活性物质研究所,广东广州510006

出　　处：《广东药学院学报》2013年第2期199-201,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省科技计划项目(2009B030801345)

摘　　要：目的通过MTT实验、迁移实验,观察Λ-WH0402对人脐静脉内皮细胞(HUVEC)增殖、迁移的抑制作用。方法将Λ-WH0402稀释为0.625、1.25、2.5、5、10、20、40、80μg/mL,MTT法观察Λ-WH0402对HUVEC细胞(Λ-WH0402敏感细胞系HCCLM6细胞作为对照)增殖的抑制作用;将不同质量浓度(0.312、0.625、1.25μg/mL)的Λ-WH0402作用于HUVEC细胞,通过迁移实验观察Λ-WH0402对HUVEC细胞迁移能力的抑制作用。结果随着Λ-WH0402浓度的增加,其对HUVEC细胞的生长抑制率显著增加,呈剂量依赖性;Λ-WH0402处理后细胞迁移活性随着药物浓度的增加明显下降。结论Λ-WH0402对HUVEC细胞增殖及其迁移能力均有抑制作用。Objective To investigate the inhibition of Λ-WH0402 on the proliferation and migration of human umbilical vein endothelial cells（HUVEC） by MTT and migration assay.Methods Λ-WH0402 was diluted to a series of different concentrations including 0.625,1.25,2.5,5,10,20,40 and 80 μg/mL.HUVEC cells were treated with different dosages of Λ-WH0402 at 0.312,0.625 and 1.25 μg/mL.The inhibition of Λ-WH0402 on HUVEC cells migration was measured by migration assay.Results The inhibition rate of Λ-WH0402 on the proliferation and migration of HUVEC cells increased significantly in a dose-dependent manner.Conclusion Λ-WH0402 can inhibit the proliferation and migration of HUVEC cells.

关 键 词：Λ-WH0402 增殖 迁移 

分 类 号：R363[医药卫生—病理学]