肝硬变患者肝胰组织中HBV DNA的分布  

HBV DNA distribution of hepatitis B virus in pancreas and liver of patients with cirrhosis

作　　者：时德仁[1] 陆立[2] 王建华 东传凌 丛闻天 

机构地区：[1]中国人民解放军201医院感染科,辽宁省辽阳市111000 [2]中国人民解放军230医院,辽宁省丹东市118000 [3]丹东市第二医院内科,辽宁省丹东市118000

出　　处：《世界华人消化杂志》2000年第7期751-754,共4页World Chinese Journal of Digestology

摘　　要：目的探讨 HBV DNA 对胰腺的致病作用.方法应用地高辛素标记 HBV DNA s 577bp 探针原位杂交(ISH)和免疫组织化学技术,检测12例血清 HBV 标志物阳性的肝硬变患者肝、胰组织石蜡包埋切片 HBV DNA 的存在.结果 ISH 采用 HBV DNA S 577bp 探针检测肝组织11例(11/12)阳性,糖耐量试验(GTT)异常7例 HBV DNA 均阳性,GTT 正常者阳性4例(4/5).胰腺组织阳性8例(8/12),GTT异常7例均阳性,GTT 正常者1例阳性(1/5),HBV DNA 阳性主要位于肝细胞、胰腺腺泡细胞和胰岛细胞的细胞核内.免疫组织化学法检测肝组织 HBsAg 均阳性,胰腺组织阳性10例(10/12),主要在胞质;而 HBcAg 在肝组织阳性5例(5/12),胰腺组织阳性5例(5/12)主要分布在细胞核,胞质分布量较少.结论 HBV 可直接侵害胰腺腺泡细胞和胰岛细胞,它可能是造成 HBV 感染后并发糖尿病的直接原因.AIM To study the pathogenesis of hepatitis B virus (HBV) DNA in pancreas tissues. METHODS HBV DNA was detected in paraffin-embedded pancreatic and hepatic tissues from 12 cirrhosis patients with positive serum HBV markers using in situ hybridization (ISH) with a digoxigenin labelled probe and immunohistochemistry was used for detection of HBV antigen. RESULTS Eleven of 12 liver specimens were HBV DNA positive,including 7 patients with abnormal glucose tolerance test (GTT) and 4 patients (4/5) with normal GTT.Eight of 12 pancreatic specimens were HBV DNA positive including 7 patients with abnormal GTT,but only one patient with normal GTT.HBV DNA was mainly localized in the nuclei of hepatic cells and pancreatic acinar and islet cells.The HBsAg distribution detected by immunohistochemical staining was positive in the cytoplasm of hepatic ceils of all patients and pancreatic cells of 10 patients,The distribution of HBcAg was positive mainly in the nuclei of hepatic cells of 5 patients and pancreatic cells of 5 patients.Faint positive distribution could be found in the cytoplasm. CONCLUSION HBV may invade the pancreatic acinar and islet cells directly,and a cause of diabetes mellitus complicated by HBV infection.

关 键 词：肝硬化 胰腺 原位杂交 免疫组织 化学 

分 类 号：R575.2[医药卫生—消化系统]