Src、HIF-1α和VEGF在大鼠脑局部缺血损伤中表达的实验研究  

Expression of Src,HIF-1α and VEGF in rat brain with experimental focal cerebral ischemia

作　　者：张振[1] 李丹阳[1] 董祥慧[1] 华威[1] 方想[1] 戚基萍[1] 

机构地区：[1]哈尔滨医科大学附属第一医院病理科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2013年第2期99-102,共4页Journal of Harbin Medical University

基　　金：国家自然科学基金资助项目(30973106);黑龙江省教育厅科学技术研究项目(12511174);哈医大一院科研基金(2012YB007)

摘　　要：目的研究大鼠脑局部永久性缺血后非受体酪氨酸激酶Src、缺氧诱导因子-1α(hypoxia induc-ible factor-1α,HIF-1α)和血管内皮生长因子(vascular endothelial growth factor,VEGF)表达及变化规律。方法通过线栓法建立大鼠大脑中动脉缺血模型,利用免疫组化检测不同出血点大脑组织Src、HIF-1α和VEGF的表达。结果 Src激酶在缺血2 h开始表达,24 h达到高峰,3天后表达开始下降,HIF-1α,VEGF表达与Src激酶表达趋于一致,三者表达成正相关。结论脑缺血后激活的Src激酶可能通过上调HIF-1α进而促进VEGF表达导致血管通透性增加。Objective To investigate the expressions of nonreceptor tyrosine kinase Src, hy- poxia inducible factor-1a （HIF-1a）, and vascular endothelial growth factor （VEGF） in rat brain tissue at different time after focal cerebral ischemia. Methods Rat middle cerebral ar- tery occlusion model was developed by suture method. The expression of Src, HIF-1a, and VEGF in rats brain was detected by immunohistochemistry. Results The express!on of Src ki- nase was detected at the time of 2 h, and reached a maximum at 24 h, then decreased after 3 days of cerebral ischemia. The expression of HIF-1a and VEGF was positively correlated with Src. Conclusion The activated Src kinase after cerebral ischemia may up-regulate the HIF- 1a＇ s expression which increases VEGF leading to the vascular permeability.

关 键 词：SRC 缺氧诱导因子 血管内皮生长因子 脑缺血 

分 类 号：R-332.4[医药卫生] R743