胃癌腹腔种植模型血清Na^+和小肠Na^+-K^+-ATP酶的表达分析  

Expression of serum sodium and intestinal Na^+-K^+-ATPase in the peritoneal implantation model of gastric cancer

作　　者：樊宁[1] 宋印利[1] 赵宝山[1] 邵畅[1] 许圣洁[1] 贺岩[1] 金晓明[1] 

机构地区：[1]哈尔滨医科大学病理学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2013年第2期117-121,共5页Journal of Harbin Medical University

基　　金：黑龙江省教育厅重点项目(12521z017)

摘　　要：目的探讨胃癌晚期肠蠕动减慢是否与血清Na+和小肠Na+-K+-ATP酶的变化有关,通过药物干预能否改善这种变化。方法 44只裸鼠,分为空白组,培养液组,模型组,用药组,下腔静脉取血测Na+及免疫荧光测小肠Na+-K+-ATP酶表达情况。结果①空白组灰度值高于3周,5周,7周模型组(P<0.01)及3周,5周培养液组(P<0.01);5周模型组灰度值高于用药3次24 h及48 h组(P<0.05,P<0.01);7周模型组灰度值低于用药组(P<0.05)。②空白组Na+高于5周,7周模型组(P<0.05,P<0.01)及3周,5周培养液组(P<0.05,P<0.01),5周模型组Na+高于用药3次24 h及48 h组(P<0.05,P<0.01),7周模型组Na+低于用药组(P<0.01)。③5周组和7周组的血Na+与小肠ATP酶的表达呈正相关,(P<0.01,P<0.05)。结论哇巴因和干细胞因子分别对Na+-K+-ATP酶有抑制和刺激作用,血清Na+的降低可能与小肠ATP酶的减少相关。Objective To investigate whether the slowed intestinal peristalsis appears in the late gastric cancer is related to the change of serum sodium and Na ＋ -K ＋ -ATPase in small intes- tine, and whether the change can be improved by interventing with drug. Methods Forty-four nude mice were divided into blank group, culture medium group, model group, administration group, and the blood Na ＋ in inferior vena cava and the expression of Na ＋ -K ＋ -ATPase in intes- tine were examined by immunofluorescence. Results ①According to gray value, the blank group was significantly higher than 3 w, 5 w, 7 w model group（P 〈0.01 ）and culture medium group （P〈0.01）, the 5 w model group was higher than 24 h group （P〈0.05） and 48 h group （P 〈0. 01 ）, and the 7 w model group was lower than administration group （P 〈0. 05）. ②The sodium value in the blank group was higher than that in 5 w, 7 w model group （P 〈 0. 05,P 〈0.01） and 3 w, 5 w culture medium group（P 〈0.05, P 〈0. 01）, the 5 w model group was higher than 24 h group （ P 〈 0. 05 ） and 48 h group （ P 〈 0. 01 ）. The 7 w model group was lower than SCF group （P 〈0. 01 ）. ③The blood sodium value of 5 w and 7 w groupwas positively correlated with Na ＋ -K ＋ -ATPase in intestine （ P 〈 0. 01, P 〈 0. 05 ）. Conclusion Na ＋ -K＋ -ATPase are stimulated and inhibited respectively by ouabain and stem cell factor. The decreased serum sodium may be related to the reduction of the small intestine Na ＋ -K ＋ -ATPase.

关 键 词：胃癌腹腔种植模型 血清Na+ NA+-K+-ATP酶 哇巴因 干细胞因子 

分 类 号：R735.2[医药卫生—肿瘤]