小单孢菌40027菌株质粒pJTU112复制区的克隆与序列特性  

Cloning and sequence characterization of replication region in plasmid pJTU112 from Micoromonospora sp.40027

作　　者：耿旭梅[1] 陈鑫[1] 杨晓潼[1] 郭佳[1] 翟贵发[1] 李晓华[1] 

机构地区：[1]中南民族大学生命科学学院,生物技术国家民委重点实验室,武汉430074

出　　处：《微生物学报》2013年第6期623-627,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31070087;30570046);湖北省自然科学基金重点项目(2011CDA079;2008CDB076);国家大学生创新创业训练项目(GCX12012)~~

摘　　要：福堤霉素A产生菌——小单孢菌40027菌株含有两个质粒pJTU101和pJTU112。【目的】对质粒pJTU112复制区进行克隆,并对质粒pJTU112复制区序列进行测定和分析。【方法】克隆质粒pJTU112的不同DNA片段导入消除质粒的小单孢菌40027菌株,通过复制功能的测定,确定质粒pJTU112的复制区,并进行测序和生物信息学分析。【结果】质粒pJTU112的复制区定位在约4.7 kb的SacI-KpnI DNA片段上,测序和生物信息学分析表明:4.7 kb的SacI-KpnI DNA片段包含5个ORFs(open reading frames),其中pJTU112.1和pJTU112.2与质粒接合转移有关,pJTU112.3、pJTU112.4和pJTU112.5与质粒复制有关。【结论】质粒pJTU112的复制区定位在约4.7 kb的SacI-KpnI DNA片段上。Micoromonospora sp.40027,the producer of fortimicin A,harbors two plasmids pJTU101 and pJTU112.[Objective] Cloning and sequencing of replication region of pJTU112,analyzing replication region sequence of pJTU112.[Methods] Different fragments of pJTU112 were cloned and introduced by conjugation into Micromonospora sp.LXH20.The replication region of pJTU112 was identified.[Results] The replication region of pJTU112 was located in the 4.7 kb SacI-KpnI DNA fragment.DNA sequencing and analysis revealed that the 4.7 kb SacI-KpnI DNA fragment encoded five open reading frames.The pJTU112.1 and pJTU112.2 were related to plasmid conjugation,pJTU112.3,pJTU112.4 and pJTU112.5 were related to plasmid conjugation.[Conclusion] The replication region of pJTU112 was located in the 4.7kb SacI-KpnI DNA fragment.

关 键 词：小单孢菌40027菌株 质粒pJTU112 复制区 克隆 

分 类 号：Q933[生物学—微生物学]