基底样型和Luminal A型乳腺癌microRNAs表达谱的生物信息学分析  

Bioinformatics-based Analysis for microRNAs Expression Profiles between Basal-like and Luminal A Breast Cancers

作　　者：姜茂竹[1] 麦仲伦[1] 曾融[1] 吴钢[1] 郑燕芳[1] 张积仁[1] 

机构地区：[1]南方医科大学珠江医院肿瘤中心,广州510282

出　　处：《肿瘤防治研究》2013年第5期417-421,共5页Cancer Research on Prevention and Treatment

摘　　要：目的对基底型和Luminal A型乳腺癌microRNAs表达谱进行分析,找出两种分子亚型乳腺癌之间差异表达的microRNAs,并对差异表达microRNAs在不同分子亚型乳腺癌中可能发挥的生物学功能进行初步探讨。方法从公共基因芯片数据库GEO中筛选基底样型和Luminal A型乳腺癌microRNAs表达谱数据,利用BRB-arrayTools软件筛选出差异表达的microRNAs。对差异表达的microRNAs,利用TargetScan和miRDB靶基因预测软件及TarBase数据库获得可能的靶基因集。利用DAVID数据库,对差异表达micorRNAs的靶基因集进行进一步GeneOntology和信号通路分析。结果通过对基底样型和Luminal A型乳腺癌microRNAs表达谱分析获得54个差异表达的microRNAs(P≤0.001)。相对于Luminal A亚型乳腺癌,31个microRNAs在基底样型乳腺癌中上调表达,而23个microRNAs下调表达。上调和下调表达microRNAs可能的靶基因集数目分别为4 916和3 217个。对于上调和下调microRN As的靶基因集,Gene Ontology分析表明,两个靶基因集分别在不同的生物学过程显著富集;KEGG通路分析分别涉及了35条和39条(P≤0.05);BIOCARTA通路分析则分别涉及5条和9条(P≤0.05)。结论本研究获得了基底样型和Luminal A型乳腺癌差异表达的microRNAs,并通过靶基因功能分析获得差异表达microRNAs在两种分子亚型乳腺癌之间可能参与的不同生物学过程及信号通路,可能在不同分子亚型乳腺癌中发挥不同的调节作用。Objective To explore differently expressed microRNAs between basal and Luminal A subtype of breast cancers and study the regulatory roles of these microRNAs.Methods A dataset of microRNAs expression profilings of basal and Luminal A subtype of breast cancers was obtained from GEO database and analyzed by BRB-ArrayTolls. Target gene sets were collected by prediction software TargetScan, miRDB and TarBase. And then Gene Ontology categories and pathways of target gene sets were further analyzed by DAVID database.Results Up-regulation of 31 microRNAs and down-regulation of 23 microRNAs were identified in basal compared with Luminal A breast cancers （P≤0.001）.Correspondingly, two gene sets of 4 916 and 3 217 target genes were collected. Further Gene Ontology analyses showed that different Gene Ontology categories were enriched between two target gene sets. There were 35 and 39 KEGG pathways （P≤0.05） were enriched separately in two target gene sets. Also, 5 and 9 BIOCARTA pathways were enriched （P≤0.05）. Conclusion There are different microRNAs expression patterns between Basal and Luminal A breast cancers.Function analysis indicated that differently expressed microRNAs may take partin quite different biological processes and signaling pathways, and may have different regulating roles in different breast cancers.

关 键 词：乳腺癌 基底样型 LUMINAL A型 微小RNAS 生物信息学 

分 类 号：R737.9[医药卫生—肿瘤]