ras癌基因诱导小鼠肝肿瘤发生及脂质代谢紊乱  

Hepatic Tumor and Lipid Metabolism Disorder Induced by ras Oncogene

作　　者：武艳霞[1,2] 董建一[1] 李慧玲[1] 汤莉[2] 王爱国[1] 王靖宇[1] 

机构地区：[1]大连医科大学实验动物中心,辽宁大连116044 [2]大连大学医学院

出　　处：《肿瘤防治研究》2013年第5期422-424,共3页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30872950)

摘　　要：目的探究ras癌基因在肝细胞中特异性表达对肝肿瘤发生及脂质代谢的影响。方法采集6～8月龄H-ras 12V转基因雄鼠的肝组织和血清,分别进行肝组织病理学检测和血清ALT、总胆固醇及甘油三酯水平的检测。结果 7月龄雄性转基因小鼠100%发生了肝肿瘤。病理学和血清学检测表明,与野生型小鼠相比,转基因小鼠的肝脏,特别是肝肿瘤,发生了显著的脂肪变性;转基因小鼠的ALT、总胆固醇水平显著升高,甘油三脂水平显著下降。结论 ras癌基因在肝细胞中的特异性表达诱导了肝肿瘤发生、肝细胞损伤和脂肪变性,并显著影响机体的脂质代谢。Objective To investigate the hepatic tumorigenesis and aberration of lipid metabolism induced by expression of H-ras12V in hepatocytes.Methods The liver tissue and serum samples of 6-8 month-age H-ras 12V transgenic mice were collected and the histopathological analysis for liver and serum examination for ALT, total cholesterol, and triglyceride levels were performed. Results 7-month H-ras12V transgenic mice developed hepatic tumor with 100% incidence. Histopathological and serum examination showed that in the liver, specially the hepatic tumor tissues of H-ras12V transgenic mice developed steatosis significantly compared with that in the wild type mice, and the serum levels of ALT and total cholesterol increased and triglyceride decreased significantly compared with those in the wild type mice.Conclusion Ras oncogene expressing specially in hepatocytes induces hepatic tumorigenesis, liver cell injury and steatosis, which influence the lipid metabolism of whole body.

关 键 词：RAS癌基因 肝癌 脂质代谢 

分 类 号：R735.7[医药卫生—肿瘤]