microRNA-93在胃癌中的表达及其临床意义  

Clinical Significance of microRNA-93 Expression in Gastric Tumor

作　　者：张广钰[1] 田小林[1] 钟漓[1] 戴凌[1] 朱袭嘉[1] 蒋志庆[1] 

机构地区：[1]桂林医学院附属医院胃肠外科,广西桂林541001

出　　处：《肿瘤防治研究》2013年第5期447-450,共4页Cancer Research on Prevention and Treatment

摘　　要：目的探讨microRNA-93在胃癌中的表达情况及其与临床病例特征的相关性。方法提取60例胃癌及其相应正常胃黏膜组织标本的总RNA,采用RT-qPCR方法检测microRNA-93的相对表达量,并分析其与临床TNM分期、淋巴结转移、分化程度以及肿瘤治疗疗效和预后等临床病理因素的关系。结果胃癌组织与癌旁正常组织比较,microRNA-93在胃癌组织中表达上调,而且其表达与TNM分期和淋巴结转移有显著相关性(P<0.05)。以相对表达量≥2.0为高表达为界限,进一步的多因素分析发现TNM分期、淋巴结转移、microRNA-93的表达与患者的预后显著相关(P<0.01)。microRNA-93的高表达降低了患者的疗效。结论 microRNA-93在胃癌组织中高表达,尤其是晚期转移性胃癌,提示microRNA-93在胃癌中是一种重要的癌基因。microRNA-93可能作为胃癌诊断和预后判断的分子标志物。Objective To investigate the expression of microRNA-93 and analyze their correlation with clinic-pathological features in gastric tumor.Methods The expression levels of microRNA-93 in 60 gastric tumors and their matched non-tumoradjacent tissue specimens were detected using stem-loop real-time reverse transcription-polymerase chain reaction （RT-qPCR）, and their correlation with clinic-pathologic features,cancer therapeutics and prognosis of gastric tumor was analyzed.Results Expression levels of microRNA-93 were significantly higher in tumor tissues than that in adjacent normal tissues （P〈0.05）. The expression of microRNA-93 was associated with TNM stage （P〈0.05） and lymphatic metastasis （P〈0.05）. The high expression of microRNA-93 group was the comparative expression more than 2.0. Multivariate analysis showed the prognosis was statistically correlated with TNM stage,lymphatic metastasis and microRNA-93 expression（P〈0.01）.The high expression of microRNA-93 reduced the effect of cancer therapeutics. Conclusion microRNA-93 is highly elevated in gastric cancer, especially in advanced and metastasized gastric cancer, suggesting microRNA-93 plays critical roles in carcinogenesis of gastric cancer. microRNA-93 might play an important role in gastric tumor and function as the potential gene therapy target for this tumor.

关 键 词：microRNA-93 胃癌 预后 TNM分期 淋巴结转移 

分 类 号：R735.2[医药卫生—肿瘤]