CD20+B和CD3+T淋巴细胞在乳腺浸润性导管癌和髓样癌中的表达及与预后的关系  

Expressions of CD20+B and CD3+ T Lymphocytes in Infiltrating Ductal Carcinoma and Medullary Carcinoma and Their Relationships with Prognosis

作　　者：张同先[1] 张巍[1] 刘芬[1] 刘寒[1] 牛昀[1] 

机构地区：[1]天津医科大学附属肿瘤医院乳腺癌防治教育部重点实验室 天津市肿瘤防治重点实验室,天津300060

出　　处：《肿瘤防治研究》2013年第5期463-467,共5页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(81172532);教育部长江学者和创新团队计划资助项目(TRT0743)

摘　　要：目的检测CD20+B、CD3+T淋巴细胞在乳腺浸润性导管癌和髓样癌中的表达分布,探讨淋巴细胞浸润与浸润性导管癌预后的关系。方法利用免疫组织化学链霉素亲和素生物素法检测128例浸润性导管癌,44例髓样癌中CD20、CD3的表达,分析表达差异与预后的关系。组间采用KruskalWallisTest,组内采用卡方检验进行分析。结果 (1)CD20+B、CD3+T这两种淋巴细胞在浸润性导管癌中分布均是瘤周多于瘤内,在髓样癌中淋巴细胞表达高于浸润性导管癌(P<0.05)。(2)浸润性导管癌中CD20+B、CD3+T淋巴细胞浸润越多,无病生存率越高(P<0.05)。(3)CD20+B淋巴细胞与组织学分级、肿瘤pTNM分期、淋巴结状态、复发情况呈负相关(P<0.05);CD3+T淋巴细胞与组织学分级、淋巴结状态、复发状态呈负相关(P<0.05)。结论 CD20+B、CD3+T淋巴细胞在浸润性导管癌和髓样癌中表达差异有显著性,在浸润性导管癌中其浸润程度与预后相关。Objective To detect the expression and distribution of CD20＋B and CD3＋T lymphocytes in breast cancer, discuss the relationship between the prognosis of breast cancer and lymphocyte infiltration.Methods Using immunohistochemistry（IHC） to investigate the expression of CD20 and CD3 in 44 medullary carcinoma cases and 128 cases of infiltrating ductal carcinoma, the relationship between the expression differences and prognosis was analyzed, Kruskal-Wallis Test was used among groups, Chi-square Test was used within the group.Results （1）There was a higher numbers of CD20＋B lymphocytes and CD3＋T lymphocytes in medullary carcinoma than that in infiltrating ductal carcinoma （P〈0.05）. Both of CD20＋B lymphocytes and CD3＋T lymphocytes located in peritumoral were more than that in intratumoral of infiltrating ductal carcinoma.（2）The higher numbers of CD20＋B lymphocytes and CD3＋T lymphocytes was associated with the longer disease-free survival in infiltrating ductal carcinoma（P〈0.05）.（3）CD20＋B ymphocytes was negatively correlated with tumor grade,pTNM,nodal stage and recurrence（P〈0.05）;CD3＋T lymphocytes was negatively correlated with tumor grade,nodal stage and ecurrence（P〈0.05）. Conclusion There are different expression of CD20＋B and lymphocytes CD3＋T lymphocytes in infiltrating ductal carcinoma,and medullary carcinoma and the lymphocytes was associated with prognosis.

关 键 词：CD20 CD3 淋巴细胞 浸润性导管癌 预后 

分 类 号：R737.9[医药卫生—肿瘤]