柯萨奇B组1型云南分离株MSH／KM9／2009全基因组序列分析  

Genomic Characteristics of Coxsackievirus B1 MSH/KM9/2009 Strain Isolated in Yunnan,China

作　　者：刘建生[1,2] 邵聪文[1,2] 潘玥[1,2] 朱艳菊[1,2] 邓新强[1,2] 廖宏玮 刘雅灵[1,2] 马绍辉[1,2] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,昆明650118 [2]云南省重大传染病疫苗研发重点实验室,昆明650118

出　　处：《病毒学报》2013年第3期293-298,共6页Chinese Journal of Virology

摘　　要：对2009年分离自云南省昆明市脑膜脑炎儿童的1株柯萨奇B组1型病毒MSH-KM9-09,进行了全基因组的序列测定,并对其分子变异和进化特点进行了分析。采用逆转录-聚合酶链反应(Reverse transcription-polymerasechain reaction,RT-PCR),分段扩增覆盖病毒全长序列的8个重叠片段(未包括多聚腺苷酸尾),并进行序列测定和分析。CVB1MSH-KM9-09病毒株基因组全长7 384个核苷酸(nt),5'端和为3'端分别为741nt和94nt的非编码区,5'和3'非编码区间为一个6 549nt长的开放阅读框,编码一个含2 183个氨基酸的多聚蛋白,编码区内未见核苷酸的插入或缺失。与GenBank库中现有CVB1全基因组序列M16560、pmMC、Tucson B1和CVB1/Nm相比对,整个基因组的核苷酸同源性分别为80.9%、81.6%、80.5%和80%,氨基酸同源性分别为95.6%、95.8%、96.2%和95.6%。首次完成中国CVB1病毒全基因组核苷酸序列的测定,经核苷酸序列同源性比对和进化树分析,证实MSH/KM9/2009株属于CVB1基因型。To characterize the complete genome sequence of coxsackievirus B1 （CVB1） MSH/KM9/2009 strain isolated from Yunnan, China,2009. Eight overlapping clones covering the whole viral genome （ex- cluding the poly-A tail） were obtained by RT-PCR and sequenced, and their nucleotide and amino acid se- quences were compared with other known CVB1 strains. The genome of the CVB1 MSH/KM9/2009 strain had 7384 nucleotides in length, and contained a 741nt non-translated region （NTR） at the 5rend and a 94nt NTR at the 3＇end. The entire open reading frame contained 6 549 nt, encoding a 2 183-aa polyprotein. In the coding region, there was no nucleotide deletion or insertion, but some changes of amino acid were u- nique. The complete genome sequence alignments showed that the CVB1 isolate MSH/KMg/2009 strain shared the highest nucleotide （80.9% ,81.6%,80.5% and 80o/oo） and amino acid （95.6% ,95.8% ,96.2% and 95.6 %） identities to the CVB1 M16560, pmMC, Tucson B1 and CVB1Nm strain, respectively. Phylo- genetic tree analysis showed that the MSH/KM9/2009, CVB1 M16560,pmMC, Tucson B1 and CVBINm strain clustered into same group. The newly isolated CVB1 strain MSH/KM9/2009 from Yunnan Province belonged to genotype CVB1.

关 键 词：柯萨奇病毒B组1型 全基因组 同源性 序列分析 

分 类 号：R373.9[医药卫生—病原生物学]