实验性糖尿病牙周炎大鼠牙周组织中基质生成细胞凋亡的初步研究  

Apoptosis of Matrix-producing Cells in the Periodontium in Streptozototocin-and Ligature-Induced Experimental Diabetic Periodontitis in Rats

作　　者：付永伟[1] 和红兵[2] 李向新[1] 龚永庆[1] 徐宏志[1] 

机构地区：[1]连云港市第一人民医院口腔科,江苏连云港222002 [2]昆明医学院附属口腔医院牙周病科

出　　处：《口腔医学研究》2013年第5期397-401,共5页Journal of Oral Science Research

基　　金：云南省应用基础研究面上资助项目(2007c218m);昆明医学院第一附属医院院内基金资助项目(2004byf14)

摘　　要：目的:观察糖尿病牙周炎条件下牙周组织中基质生成细胞的凋亡情况。方法:选用6周龄雄性SD大鼠62只,随机分为糖尿病牙周炎组(DP)、牙周炎(P)以及正常对照组(N)。DP组采用一次性腹腔注射链脲佐菌素方法诱导大鼠糖尿病模型后,采用丝线结扎法建立牙周炎模型。P组仅建立牙周炎模型,N组不予处理。实验动物分别于P组和DP组丝线结扎后3、6周分批处死,进行牙槽骨丧失的测量和组织学检测。观察指标:牙槽骨丧失、单位面积牙周膜中成纤维细计数、单位长度牙槽骨表面成骨细胞计数、牙周膜中成纤维细胞凋亡率以及牙槽骨表面成骨细胞凋亡百分率。结果:与N组比较,P组和DP组牙槽骨丧失明显增加(P<0.05),与P组比较,DP组牙槽骨丧失亦显著增加(P<0.05)。与N组比较,P组和DP组成纤维细胞及成骨细胞数目减少,与P组比较,DP组成纤维细胞及成骨细胞数目显著减少(P<0.05)。与N组比较,P组和DP组凋亡成纤维细胞及凋亡成骨细胞增加,与P组比较,DP组凋亡成纤维细胞及凋亡成骨细胞增加,有统计学意义(P<0.05)。结论:在牙周炎条件下,糖尿病可能通过加强牙周组织中基质生成细胞的凋亡,降低这些细胞的数量,从而促进牙周组织的破坏。Objective： To investigate the presence of apoptosis of matrix-producing cells in the periodontium in streptozototocin- and ligature-induced experimental diabetic periodontitis in rats. Methods： 62 six-week-old male Sprague-Dawley （SD） rats were randomly divided into： diabetic periodontitis group （group DP）, periodonti tis group （group P）, and normal control group （group N）. Diabetes was induced by intraperitoneal injection of streptozototocin （STZ）. Periodontitis was initiated by ligating floss around maxillary second molars. In group DP, periodontitis was induced following induction of diabetes. In group P, periodontitis was induced alone. In group N, animals were left untreated. The animals were sacrificed at 3 and 6 weeks after ligature placement in P and DP groups. Maxillary dentoalveolar segments were isolated and were prepared for morphometric analysis of alveolar bone loss （ABL） and for histological analysis. Results： ABL measurements revealed significantly higher values in group DP and group P （P〈0.05）. ABL was significantly increased in group DP compared with group P （P〈0. 05）. Intergroup comparisons demonstrated that periodontitis and diabetic periodontitis might reduce the number of PDL fibroblasts and osteoblasts. Moreover, the number of PDL fibroblasts and osteoblasts in group DP was also decreased compared with group P （P 〈 0.05）. Periodontitis and diabetic periodontitis also increased apoptosis of fi broblasts and osteoblasts. The percentage of these apoptotic cells was approximately 2-fold higher in group DP vs. group P. Conclusion： The results suggest that diabetes may have a negative effect on the periodontium by leading to greater apoptosis and reduced numbers of matrix-producing cells.

关 键 词：牙周炎 糖尿病 动物模型 凋亡 

分 类 号：R781.4[医药卫生—口腔医学]