乳果糖预处理减轻大鼠肠缺血再灌注损伤的机制研究  

Lactulose preconditioning protects against intestinal ischemia-reperfusion injury in rats

作　　者：叶志强[1] 戴海涛[1] 刘旭辉[1] 曾花[1] 邢帮荣[1] 陈锐涵[1] 卫洪波[2] 

机构地区：[1]中山大学附属第三医院急诊科,广东广州510630 [2]中山大学附属第三医院胃肠外科,广东广州510630

出　　处：《中国病理生理杂志》2013年第5期918-922,共5页Chinese Journal of Pathophysiology

基　　金：广东省科技计划项目(No.2011B061300011)

摘　　要：目的:探讨乳果糖预处理对大鼠肠缺血再灌注损伤的影响及其作用机制。方法:随机将30只SD大鼠分成假手术组、缺血再灌注组和乳果糖预处理组。乳果糖预处理组在手术前7 d每天给予乳果糖灌胃,假手术组和缺血再灌注组在手术前7 d每天给予等量生理盐水灌胃。手术分离肠系膜上动脉,通过夹闭30 min、再灌注60 min诱导缺血再灌注损伤。收集血清检测白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)和IL-1β水平。HE染色用来评估组织的损伤程度,TUNEL检测小肠上皮细胞的凋亡。部分小肠组织用来检测丙二醛、超氧化物歧化酶及激活型caspase-3的表达水平。结果:乳果糖预处理显著减轻缺血再灌注引起的肠组织损伤和小肠上皮细胞凋亡,并显著抑制血清中细胞因子的水平和肠组织的脂质过氧化。结论:乳果糖预处理可能通过抑制细胞凋亡和脂质过氧化减轻缺血再灌注引起的肠道损伤。AIM： To investigate the protective effect of lactulose preconditioning on intestinal ischemia-reperfu- sion （IR） injury in rats. METHODS： Thirty Sprague-Dawley rats were randomly divided into 3 groups： sham operation group, IR group and IR plus lactulose preconditioning group. Lactulose was intragastrically administered in lactulose group 7 days prior to operation, and the equal volume of saline was administered in the other 2 groups. The intestinal IR injury was induced in IR group and IR ＋ lactulose group using bulldog clamps on superior mesenteric artery by 30 min of ischemia followed by 60 min of reperfusion. Following repeffusion, the serum samples were collected for estimating the levels of in- terleukin 6 （IL-6）, tumor necrosis factor a （TNF-a） and IL-1 β. Segments of terminal jejunum were rapidly fixed in 4% paraformaldehyde, and HE staining was applied to assess the histopathology. Apoptosis in intestinal epithelium was deter- mined by the technique of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling （TUNEL）. The samples of terminal jejunum were also taken for measuring malondialdehyde, superexide dismutase and the expression of cleaved caspase-3. RESULTS： Lactulose preconditioning significantly attenuated the severity of intestinal IR injury, with inhibition of IR-induced apoptosis. Moreover, lactulose preconditioning significantly limited the release of cytokines and lipid oxidation. CONCLUSION： Lactulose preconditioning has a protective effect on intestinal ischemia reperfusion by in- hibiting IR-induced apoptosis and oxidative stress.

关 键 词：肠缺血再灌注损伤 乳果糖 

分 类 号：R363[医药卫生—病理学]