冬虫夏草口服液游离氨基酸和微量元素的分析比较  

Analysis and Comparison of Free Amino Acid and Trace Element from Oral Solution of Cordyceps sinensis

作　　者：汪家春[1] 陈雅琳[1] 杨睿倩[2] 

机构地区：[1]中国人民解放军海军医学研究所,上海200433 [2]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《生物技术通讯》2013年第3期393-397,共5页Letters in Biotechnology

基　　金：解放军总后勤部科研项目

摘　　要：目的:为了研究冬虫夏草口服液的有效成分和药用价值,测定冬虫夏草口服液中的游离氨基酸和微量元素的不同种类和含量。方法:采用安捷伦1200高效液相色谱仪,C18反相柱梯度分离,邻苯二甲醛-芴甲酯柱前自动衍生化,利用已知浓度氨基酸标准品进行外标法定量,根据已知浓度氨基酸标准品的峰面积与样品峰面积之比计算游离氨基酸的含量。结果:冬虫夏草口服液中含有23种游离氨基酸和18种微量元素,氨基酸总量在口服液中占0.892%,必需氨基酸在游离氨基酸总量中占53.1%;含量最高的游离氨基酸依次是赖氨酸、亮氨酸、精氨酸。结论:游离氨基酸是冬虫夏草口服液中重要的生物有效成分,为冬虫夏草口服液的药效提供了基础数据。Objective： In order to research on the active ingredient and medicinal value of Cordyceps sinensis, free amino acids and trace elements of C.sinensis oral solution were determined. Methods： A C18 column was used with gradient elution by RP-HPLC and OPA-FMOC pre-column derivatization. The external standard method was used to quantify the amino acids by the peak areas ratio between the sample and the standard. Results： The analysis showed that C.sinensis oral solution contained 23 kinds of free amino acids and 18 kinds of trace elements. The total amount of amino acid in the oral solution was 0.892%, and the essential amino acids to total amino acids was 53.1%. Content of lysine was the most high, followed by leucine and arginine, among all kinds of amino acids in the oral solution. Conclusion： Free amino acids are bioaetive ingredients of C.sinensis oral solution,which supply basic data for the efficacy of C.sinensis oral solution.

关 键 词：冬虫夏草 游离氨基酸 微量元素 名贵中药材 

分 类 号：Q503[生物学—生物化学] R917[医药卫生—药物分析学]