不同转移能力的卵巢上皮性癌细胞中matriptase mRNA和蛋白的表达及与其转移能力的相关性  

Expression and significance of matriptase in ovarian cancer cells with diverse metastatic potential

作　　者：江忠清[1] 陈小芳[1] 孙蓬明[2] 毛晓丹[2] 林芬[1] 宋一一[2] 

机构地区：[1]福建医科大学附属第一医院妇科,福州350005 [2]福建省妇幼保健院妇科

出　　处：《中华妇产科杂志》2013年第5期370-374,共5页Chinese Journal of Obstetrics and Gynecology

基　　金：福建省科技计划（2009Y0007）;福建省自然科学基金（2012J01310）

摘　　要：目的研究不同转移能力的卵巢上皮性癌（卵巢癌）细胞中胰蛋白酶样Ⅱ型跨膜丝氨酸蛋白酶（matriptase）的表达，并探讨其与卵巢癌细胞转移能力的相关性。方法选取卵巢癌细胞系H08910及其配对高转移能力细胞系H08910PM，应用体外划痕实验及穿膜（transwell）小室法检测两种细胞的体外迁移及侵袭能力，应用逆转录（RT）-PCR和细胞免疫荧光技术检测两种细胞中matriptase mRNA和蛋白的表达水平，并对卵巢癌细胞中matriptasemRNA和蛋白的表达水平与其体外迁移、侵袭能力的相关性进行Pearson相关性分析。结果体外戈4痕实验最示，划痕后培养24h，H08910PM细胞的迁移距离为（347±8）μm，明显大于H08910细胞的（154±10）灿m（P〈0．01）；穿膜小室法检测显示，24h后H089IOPM细胞的穿膜细胞数为（90．7±2．1）个，明显高于H08910细胞的（63．3±1．5）个（P〈0．01）。RT—PCR技术检测显示，H08910PM细胞巾matriptase mRNA的表达水平为0．72±0．03，明显高于H08910细胞的0．38±0．04（P〈0．01）；细胞免疫荧光技术检测显示，H08910PM细胞叶1matriptase蛋白的表达水平为15．6±0．8，明显高于H08910细胞的7．6±1．3（P〈0．01）。Pearson相关性分析显示，卵巢癌细胞中matriptase mRNA和蛋白的表达水平与其迁移（r=0．992，r=0．971；P〈0，01）及侵袭（r=0．973，r=0．958；P〈0．01）能力均呈明显正相关。结论高转移能力的卵巢癌细胞系H08910PM细胞中matriptase mRNA和蛋白的表达水平均明显高于低转移能力的卵巢癌细胞系H08910细胞，matriptase基因的表达与卵巢癌细胞的迁移和侵袭能力可能存在相关性，其机制有待进一步研究。Objective To study the expression and significance of matriptase in different metastatic potential of human ovarian cancer cells. Methods High-metastatic human ovarian cancer cell HO8910PM and ovarian cancer eell HO8910 were collected. The ability of metastatic of the former was stronger than that of the latter. Compared the ability of invasion and migration in HO8910PM and HO8910 by scratch assay and by millicell chamber artificial reconstituted basement membrane invasion assay. Detected the matriptase mRNA and protein expression levels in HO8910PM and HO8910 through reverse transcription （RT）-PCR and immunocytochemistry methods. Results The 24 hours＇ migration distance（347 ± 8） μm of HO8910PM cells were significantly higher than that in HO8910 group （ 154 ± 10） μm （P 〈 0. 01 ） ; The number of HO8910PM cells that penetrated the matrigel after 24 hours＇ incubation were significantly higher than that in HO8910 group （90. 7 ±2. 1 vs 63.3 ± 1.5 ,P 〈0. 01 ）. The expression of matriptase mRNA in HO8910PM cells was higher than that in HO8910 group （0. 72 ± 0.03 vs 0, 38 ± 0. 04, P 〈 0. 01 ）. The migration was positively con＇elaled with the matriptase mRNA expression levels （r = 0.992, P 〈 0.01 ）;and the invasiveness was also positively correlated with the matriptase mRNA expression levels （ r = 0. 973, P 〈 0. 01 ）. As far protein level,the expression of matriptase protein in HO8910PM cells was higher than that in H08910 group （ 15.6 ± 0.8 vs 7.6 ± 1.3 ,P 〈0. 01 ）. The migration was positively correlated with matriptase protein expression levels （r = 0. 971, P 〈 0. 01 ） ;And the invasiveness was also positively correlated with the matriptase protein expression levels （ r = 0. 958, P 〈 0.01 ）. Conclusions The relationship between the expression levels of matriptase and the metastatic of ovarian cancer cells may be cmTelative. The function of matriptase in ovarian cancer cells metastatic machanism still need to be confirmed.

关 键 词：卵巢肿瘤 肿瘤转移 丝氨酸内肽酶类 

分 类 号：R737.31[医药卫生—肿瘤]