EpCAM和E-cadherin在膀胱尿路上皮癌的表达及意义  

Clinicopathologic Significance of EpCAM and E-cadherin Expression in Urothelial Carcinoma of Bladder

作　　者：单立平[1] 张墨[1] 李波[1] 吴斌[1] 宋永胜[1] 

机构地区：[1]中国医科大学附属盛京医院泌尿外科m辽宁沈阳110004

出　　处：《天津医药》2013年第6期527-530,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30901480);辽宁省沈阳市科学技术计划项目(项目编号:f11-264-1-64)

摘　　要：目的探讨上皮特异性黏附分子(EpCAM)和E-钙黏素(E-cadherin)在膀胱癌中的表达及临床意义。方法应用免疫组织化学SP法检测156例不同级别膀胱癌组织和80例癌旁正常组织中EpCAM和E-cadherin的表达,分析它们在不同临床病理因素膀胱癌中的表达情况,及两者在膀胱癌发展过程中的相互关系。结果膀胱癌组织中EpCAM表达高于癌旁组织(53.2%vs13.8%,P<0.01),膀胱癌组织中E-cadherin表达低于癌旁组织(35.9%vs56.3%,P<0.01)。高分级膀胱尿路上皮癌标本中可见较为典型的EpCAM高表达,低分级膀胱尿路上皮癌中可见典型E-cadherin阳性表达。EpCAM在肌层浸润性(pT2、pT3或pT4)、高分级、合并远处转移、多发及复发膀胱肿瘤中表达上调(P<0.05),E-cadherin在肌层浸润性、高分级、合并远处转移、多发膀胱肿瘤中表达下调(P<0.05)。膀胱癌中EpCAM与E-cadherin的表达呈负相关(r=-0.607,P<0.01)。结论 EpCAM和E-cadherin与膀胱癌的分化、浸润转移等恶性行为有关,两者联合检测对判断肿瘤侵袭转移能力具有一定价值。Objective To investigate the expressions of the epithelial cell adhesion molecule （EpCAM） and E-cadherin and their clinical significance in urothelial carcinoma of bladder. Methods The expressions of EpCAM and E-cadherin were analyzed by immunohistochemistry using a monoclonal antibody EpCAM and E-cadherin in 80 cases of normal mucosa and 156 cases of urothelial carcinoma of bladder. The expression levels of EpCAM and E-cadherin were analyzed in different clinical pathological factors. Moreover, EpCAM expression was investigated by comparison with E-cadherin. Results The positive rate of EpCAM expression in bladder urothelial carcinoma was 53.2%, which was significantly higher than that in para-cancerous mucosa （13.8%, P 〈 0.01）. The positive rate of EpCAM expression vas significantly lower in bladder urothelial carcinoma than that in para-cancerous mucosa （35.9% vs 56.3%, P 〈 0.01）. The positive expression of EpCAM was detected in high-grade urothelial carcinoma. The positive expression of E-cadherin was detected in low-grade urothelial carcinoma. The expression levels of EpCAM increased with the increased clinical stages, pathological grades, tumor quantity, distant metastasis and tumor recurrence （all P〈 0.05）. The expression levels of E-cadherin decreased with the increased clinical stages, pathological grades, tumor quantity and distant metastasis （all P〈 0.05）. There was a negative correlation between expressions of EpCAM and E-cadherin in bladder urothelial carcinoma （r =-0.607, P 〈 0.01）. Conclusion The expressions of EpCAM and E-cadherin are associated with tumor malignancies, such as invasion and metastasis. The evaluation of the two markers has a certain value for determining tumor invasion and metastasis.

关 键 词：膀胱肿瘤 癌 钙黏着糖蛋白类 细胞黏附分子 免疫组织化学 上皮特异性黏附分子 钙黏素 

分 类 号：R734.2[医药卫生—肿瘤]