胰岛素样生长因子-1参与诱导小鼠诱导多潜能干细胞的研究  

Roles of Insulin-like Growth Factor-1 in the Induction of Murine Induced Pluripotent Stem Cells

作　　者：闫秋月[1] 李珍[1] 胡文涛[1] 方瑜[1] 王淑楠[1] 邱占东[1] 张苏明[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《神经损伤与功能重建》2013年第3期157-159,共3页Neural Injury and Functional Reconstruction

基　　金：国家自然科学基金资助项目(No.81271407);中国博士后科学基金资助项目(No.20110490453)

摘　　要：目的:观察胰岛素样生长因子-1(IGF-1)对小鼠诱导多潜能干(iPS)细胞诱导效率的影响。方法:将鼠胚成纤维细胞分为对照组和实验组,2组均予经典四因子的逆转录病毒载体感染,实验组在培养基里加入IGF-1,进行iPS细胞诱导,并对其进行鉴定。结果:对照组和实验组的iPS细胞诱导效率分别为(0.05±0.0002)%和(0.01±0.0005)%,所获iPS细胞为阳性。结论:IGF-1提高小鼠iPS细胞的诱导效率。Objective： To study the effect of insulin-like growth factor-1 （IGF-1） on the induced efficiency of murine induced pludpotent stem（iPS） cells. Methods： Mouse embryonic fibroblasts were divided into control group and experimental group. The 2 groups were infected with retro-viruses expressing classic 4 factors while the medium of the experimental group was added IGF-1. Then the clones were determined. Result： The in- duced efficiency of the control group and the experimental group were （0.05 ±0.0002）% and （0.01 ±0.0005）% respectively. The clones were positive. Conclusion： IGF-1 improve the inducing efficiency ofiPS cells.

关 键 词：胰岛素样生长因子-1 诱导多潜能干细胞 细胞重编程 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]