大鼠局灶性脑缺血时细胞凋亡与c-fos表达  

Apoptosis and altered expression of c-fos following focal cerebral ischemia-reperfusion in rats

作　　者：张玲[1] 杨益阶[1] 余绍祖[1] 张兆辉[1] 喻文莉[1] 

机构地区：[1]湖北医科大学附属第一医院神经内科,武汉430060

出　　处：《卒中与神经疾病》2000年第2期71-74,共4页Stroke and Nervous Diseases

摘　　要：目的 :研究凋亡细胞在脑缺血及再灌注后的时空表达方式及原癌基因 c-fos的表达 ,以期探讨脑缺血时 c-fos表达与细胞凋亡的关系。方法 :用线栓法建立局灶性脑缺血再灌注模型 ,DNA缺口末端标记法原位检测细胞凋亡 ,免疫组化检测 c-fos的表达。结果 :缺血侧鼠脑凋亡细胞数随再灌注时间延长而增多 ,凋亡细胞主要分布于额顶叶皮层与纹状体 ,以梗死灶边缘区密度最高。正常组、假手术组未见 c-fos表达 ,缺血及再灌注各组缺血对侧有少量 c-fos表达 ,缺血侧 c-fos阳性细胞数随再灌注时间延长而增多。 c-fos阳性细胞主要分布于缺血侧新皮层与纹状体 ,扣带回皮层、部分丘脑与海马也见较多表达。结论 :脑内缺血性神经元凋亡可能与Objective : TO Assess the effects of focal ischemiareperfusion on the expression of cfos which have been implicated in the regulation of programmed cell death. Methods: Focal cerebral ischemia was induced in male Wistar rats(n=25) using an intraluminal monofilament blockade of the MCA.Coronal brain sections was analyzed, using an in situ Cell Death detection kit,hematoxylin and eosin, and immunohistochemical methods. Results: DNA Fragmentation was present in low level(<8%)in each hemisphere of normal and shamoperated rats as well as in the contralateral hemisphere of ischemia rats. The number of cells exhibiting apoptosis increased from 2±4.47 at 4h of ischemia without eperfusion to 31±1.87 after 2h of reperfusion and to 45±2.79 after 24h of reperfusion (P<0.05).Groups of cells exhibiting apoptosis were located primary in the frontalpariential cortex and stiatum. DNA fragmentatio was most intense in the boundary zone of the infarct.At the same time,immunohistochemical analysis revealed of cfos in the infarcted neocortes and striatum,cingulate cortes,part of thalamus and hippocampal.In infarcted hemisphere,the cfos positive cell unmber at different time point wewe 14±2.59,22±3.32,30±5.12,respectively(P<0.05).Conclusion:The findings suggest that activation of cfos may contribute to neuronal apoptosis following ischemicreperfusion injury.

关 键 词：脑缺血 再灌注 凋亡 C-FOS 

分 类 号：R743.302[医药卫生—神经病学与精神病学]