亚低温在急性脑缺血中对氨基酸和自由基含量的影响  

Effect of mild hypothermia during cerebral ischemia and reperfusion in rat

作　　者：胡波[1] 梅元武[1] 邱小鹰[2] 孙圣刚[1] 

机构地区：[1]同济医科大学附属协和医院神经科,430022 [2]桂林医学院附属医院神经科

出　　处：《卒中与神经疾病》2000年第2期75-77,共3页Stroke and Nervous Diseases

摘　　要：目的 :研究亚低温状态下 ,急性脑缺血时皮质内四种氨基酸和自由基含量的改变 ,探讨亚低温对缺血脑组织的保护机制。方法 :将 63只 Wistar大鼠随机分为 9组 ,假手术组 7只 ,缺血组 (手术后将其颞肌温度控制在 36.5± 0 .5℃ ) -缺血 3h组 ,缺血 3h分别再灌注 1 h、2 h、3h组各 7只 ;亚低温组 (手术后将其颞肌温度控制在 32 .5± 0 .5℃ )一缺血 3h,缺血 3h分别再灌注 1 h、2 h、3h组各 7只。动物模型参照 Zea longa的大脑中动脉线栓模型并加以改进。结果 :比较缺血组和低温组 ,可见低温组缺血皮质内的谷氨酸 (Glu)、天门冬安酸 (Asp)、丙二醛 (MDA)的产生明显减少 ,而超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH-PX)的生成和释放明显增加 ,γ-氨基丁酸(GABA)的含量亦增加。结论 :亚低温状态下Objective:To evaluate the effect of mild hypothermia to four types amino acids and free radial system during cerebral ischemia and repurfusion.Metheods: Sixtythree Wistat rats were divided randomly into nine groups:control group(n=7),ischemia groupsis3h,is3h/rep1h,is3h/rep3h(each group,n=7),mild hypothermia groups(10mg/kg i.p.)is3h,is3h/rep1h,is3h/rep2h,is3h/rep3h(each group,n=7)with Zea longa's animal middle of cerebral atrery occlusion.Results:Compared with the ischemia groups ,we found the concentrations of glutamate,aspartate,MDA in cerebral cortex decrease markedly and GABA,SOD,GSHPX increased significantly in mild hypothermia groups.Conclusion:It indicates that the change of concentrations of four type animo acids and free radial system is an important protective mechanism to ischemia neurons under mild hypothermia state.

关 键 词：亚低温 兴奋 抑制性神经递质 自由基 脑缺血 

分 类 号：R743.3[医药卫生—神经病学与精神病学]