兴奋性氨基酸、氧自由基与缺血再灌注脑损伤关系的实验观察  

Experimental observation on relation of excitatory amino acid,oxygen free radicals and cerebral ischemic reperfusion damage

作　　者：刘煜敏[1] 徐仁伵[1] 黄怀钧[1] 王凤霞[1] 

机构地区：[1]湖北医科大学附属第二医院神经科,430071

出　　处：《卒中与神经疾病》2000年第2期98-100,共3页Stroke and Nervous Diseases

摘　　要：目的 :观察脑缺血再灌注过程中兴奋性氨基酸 (Excitatory amino acid,EAA)、氧自由基的变化 ,研究探索脑缺血再灌注损伤的机制。方法 :测定假手术组、缺血 30 min再灌注 60 min生理盐水 (NS)处理组和单唾液酸四已糖神经节苷脂 (GM1 ,1 0 mg/ kg,IP)处理组 ,鼠脑海马组织 EAA、丙二醛 (Malondiadehyde,MDA)的含量。实验应用全脑缺血 (4 VO)模型 ,EAA采用 HPLC法 ,MDA采用 TBA法。结果 :脑缺血再灌注 NS处理组海马组织 EAA含量明显较假手术组降低 (P<0 .0 1 ) ,MDA含量则显著性升高 (P<0 .0 1 ) ,GM1 处理组 EAA,MDA含量均无明显变化 (P>0 .0 5)。结论 :EAA。Objective :To ovserve the excitatory amino acid(EAA), oxygen free radicals change on cerebral ischemic reperfusion ,and study the mechanism of cerebral ischemic reperfusion damage.Methods:The hippocampus EAA,malondiadehyde (MDA) contents of sham operated group,ischemia 30 min reperfusion 60 min normal sodium (NS) and GM 1treated group GM 1,10mg/kg,IP) were measured,the global cerebral ischemic reperfusion model (4VO) was made on the experiment,EAA was measured by high performance liquid chromatogrphic detemination (HPLC),MDA was measured by TBA. Results:Compared with sham operated group,the hippocampus EAA content of cerebral ischemic reperfusion NStreated group decreases significantly (P<0.01),MDA content increases significantly (P<0.01),the hippocampus EAA and MDA contents of GM 1treated group don't change significantly (P>0.05). Conclusion:EAA,oxygen free radicals are closely related with the mechanism of cerebral ischemic reperfusion damage.

关 键 词：兴奋性氨基酸 氧自由基 缺血再灌注脑损伤 

分 类 号：R743[医药卫生—神经病学与精神病学]