牛源性肠炎沙门氏菌的三型分泌系统基因分析  

GENE ANALYSIS OF SALMONELLA ENTERITIDIS ON THREE TYPE SECRETE SYSTEM FROM CALVES

作　　者：付红蕾 李彦 杜冬冬[2] 庄燕飞[2] 常维山[2] 王涛[3] 

机构地区：[1]青岛市饲料兽药检测站,山东青岛266071 [2]山东农业大学,山东泰安271018 [3]秦皇岛出入境检验检疫局,河北秦皇岛066000

出　　处：《山东农业大学学报（自然科学版）》2013年第2期181-184,共4页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：青岛市公共领域科技支撑计划项目(09-1-1-93-nsh)

摘　　要：肠炎沙门氏菌是一类引起人与动物腹泻的病原菌,其致病性与三型分泌系统密切有关。本次试验研究临床上腹泻牛体内分离的一株肠炎沙门氏菌致病性与三型分泌系统的关系,试验选取SipA、SipD的基因序列进行测序分析。本次试验分别运用特异性引物扩增SipA、SipD的基因,连接到T载体上并测定目的片段序列。测出的序列在GeneBank上进行比对,以确定分离菌与其他菌株亲缘关系。试验结果显示该株肠炎沙门氏菌的SipA基因、SipD基因与肠炎沙门氏菌P125109同源性达100%。Salmonella enteritidis was a kind of pathogenic bacteria causing human and animal diarrhea. The pathogenicity of it was closely related to type three secretion system. The study was aimed at the relationship be- tween salmonella enteritidis separating from clinical diarrhea cow and the type three type secretion system. SipA and SipD gene were sequenced and analysed. We used specific primer to amplify SipA and SipD gene, connect- ed T vector and determined the target fragment sequences. The measured sequences were compared in Geneltank to identify the relationship between the isolated bacteria and others. The test results showed that the the se- quences of the strains of salmonella enteritidis SipA and SipD gene corresponded with salmonella enteritidis p125109 up to 100%. The study showed that the higher homology between SipA and SipD gene of type three se- cretiong system, the more similar of pathogenicity.

关 键 词：肠炎沙门氏菌 三型分泌系统 基因序列 

分 类 号：S633.4[农业科学—蔬菜学]