表阿霉素免疫纳米微粒的制备及体外抗肿瘤作用研究  

Preparation and in vitro antitumor action research of epirubicin immunological nanoparticles

作　　者：杨峰[1] 黄国祥[1] 袁淑仪[2] 庞泓[1] 王金林[1] 龚时文[1] 

机构地区：[1]广东省东莞市人民医院普外科,广东东莞523126 [2]广东省东莞市人民医院手术室,广东东莞523126

出　　处：《中国当代医药》2013年第16期77-79,共3页China Modern Medicine

基　　金：广东省东莞市医疗卫生科技计划一般项目(201210515001108)

摘　　要：目的制备表阿霉素免疫纳米微粒,观察其抗体活性、体外释药及体外抗肿瘤作用。方法利用聚电解质复合法合成载表阿霉素纳米微粒(E-ADM-NPs),化学交联法合成载表阿霉素的抗血管内皮生长因子受体2(VEGFR2)单克隆抗体纳米微粒。ELISA法检测表阿霉素单克隆抗体纳米微粒(E-ADM-Ab-NPs)的抗体活性,紫外分光光度计测定其体外释药量,MTT法检测其对人肝癌细胞的体外杀伤效应。结果 E-ADM-Ab-NPs的平均粒径为(190±21)nm,抗体活性保存良好;体外释药试验表明,E-ADM-Ab-NPs具有缓释特性,10d累积释药量可达93.46%;E-ADM的体外杀伤效应在1～6d呈时间依赖性,而E-ADM-Ab-NPs则在1～10d均呈时间依赖性,6d时两者的杀伤效应均呈剂量依赖性,且两者间差异无统计学意义(P>0.05)。结论 E-ADM-Ab-NPs具有药物缓释效应和免疫活性,可延长表阿霉素(E-ADM)对人肝癌细胞的有效作用时间,且并未影响E-ADM的生物学活性。Objective To prepare the epirubicin immunological nanoparticles, and observe the antibody activity, drug release in vitro and antitumor effect in vitro. Methods Epirubicin immunological nanoparticles （E-ADM-NPs） was synthetized by using the polyelectrolyte complex method, anti-vascular endothelial growth factor receptor 2 （VEGFR2） monoclonal antibody nanoparticles （E-ADM-Ab-NPs） of epirubicin was synthetized by using chemical crosslinking method. E-ADM-Ab-NPs antibody activity was detected by ELISA method, its in vitro release dosage was determined by ultraviolet spectrophotometer, the in vitro killing effect of human liver cancer cells was detected by MTT method. Results The average particle size of E-ADM-Ab-NPs was （190 ± 21） nm, antibody activity was in a good condition; In vitro drug release test showed that, E-ADM-Ab-NPs had the slow-release properties, cumulative release dosage for 10 days was 93.46%; E-ADM in vitro killing effect took on time dependence in 1-6 d, and E-ADM-Ab-NPs took on time dependence in 1 -10 d, killing effects of both in 6 d took on dose dependent, and there was no statistically significant difference between them （P 0.05）. Conclusion E-ADM-Ab-NPs has drug sustained release effect and immune activity, and can prolong E -ADM of the action time to human liver cancer cells, and does not affect the biological activities of E-ADM.

关 键 词：表阿霉素 载药纳米微粒 聚电解质复合法 缓释作用 血管内皮生长因子 肝癌 

分 类 号：R730.5[医药卫生—肿瘤]