流动注射-分光光度法同时测定食品中水溶性总糖和还原糖  

Simultaneous Determination of Soluble Total Sugar and Reducing Sugar in Food by FI-Spectrophotometry

作　　者：王丽平[1] 肖靖泽[1] 赵萍[1] 

机构地区：[1]北京吉天仪器有限公司,北京100015

出　　处：《理化检验（化学分册）》2013年第5期516-520,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：食品中水溶性总糖经1mol.L-1盐酸溶液水解生成还原糖,对羟基苯甲酸酰肼与生成的还原糖显色,在波长420nm处有最大吸收峰,据此提出了一种流动注射-分光光度法测定食品中水溶性总糖和还原糖含量的方法。方法的线性范围为50.0～800mg.L-1,还原糖的检出限为5.32mg.L-1,总糖的检出限为2.71mg.L-1。方法用于食品中还原糖和水溶性总糖的测定,测得方法的回收率在98.0%～101%之间,测定值的相对标准偏差(n=6)在1.1%～1.5%之间。It was found that the soluble total sugar in food was hydrolyzed to reducing sugar with 1 mol ·L^-1 HCI solution, and a colored complex was formed by its reaction with p-hydroxybenzoic acid hydrazide, having its absorption maximum at the wavelength of 420 nm. Based on these facts, the determination of soluble total sugar and reducing sugar in food by flow injection （FI）-spectrophotometry was proposed. Linearity range was kept between 50.0 and 800 mg ·L^-1, with detection limits （3s） of 5.32 mg·L^-1 （for reducing sugar） and 2. 71 mg ·L^-1 （for soluble total sugar）. The proposed method was used in the determination of soluble total sugar and reducing sugar in food, and values of recovery found were in the range of 98.0%-101% with RSD＇s （n=6） in the range of 1.1%- 1.5%.

关 键 词：流动注射-分光光度法 还原糖 水溶性总糖 食品 

分 类 号：O657.32[理学—分析化学]