丹参酮ⅡA抑制非小细胞肺癌NCI-H520细胞生长的研究  

Tanshinone ⅡA inhibits non-small cell lung cancer NCI-H520 cells

作　　者：简晓顺[1] 刘相富[2] 陈健清[1] 

机构地区：[1]广州医学院附属肿瘤医院药学部,广州510095 [2]中山大学附属第三医院输血科,广州510630

出　　处：《新医学》2013年第5期347-350,共4页Journal of New Medicine

基　　金：广东省医学科研基金(B2012171)

摘　　要：目的:探讨丹参酮ⅡA抑制非小细胞肺癌NCI-H520细胞生长及其分子机制。方法:用不同浓度的丹参酮ⅡA(0,5,10,20μM)处理非小细胞肺癌NCI-H520细胞后,采用MTT法检测非小细胞肺癌NCI-H520细胞活力;采用流式细胞计检测非小细胞肺癌NCI-H520细胞周期;应用免疫印迹方法检测蛋白表达水平。结果:丹参酮ⅡA显著抑制非小细胞肺癌NCI-H520细胞生长,并呈浓度依赖方式;流式细胞检测到丹参酮ⅡA处理非小细胞肺癌NCI-H520细胞后,细胞周期S期细胞比例较处理前显著增多(P<0.05);免疫印迹实验检测到丹参酮ⅡA处理非小细胞肺癌NCI-H520细胞后,细胞周期蛋白Cyclin E及CDK2抑制剂P27较处理前显著上调(P<0.05)。结论:丹参酮ⅡA可能通过上调P27抑制CDK2,导致非小细胞肺癌细胞NCI-H520细胞周期S阻滞。Objective： To explore the molecular mechanism by which Tanshinone Ⅱ A （Tan Ⅱ A） inhibits non-small cell lung cancer NCI-H520 cells. Method： After dealing with different concentrations of Tanshinone Ⅱ A （0, 5, 10, 20μM）, the viability of NCI-H520 cells treated with Tanshinone Ⅱ A was detected by MTF as- say ： Cell cycle profile of NCI-H520 cells induced by Tanshinone Ⅱ A was examined by Flow Cytometry ： Immunob- lotting was utilized to determine proteins expression level. Result： Tanshinone Ⅱ A remarkably detriments NCI- H520 cells viability in dose-dependent manner： cell cycle analysis indicated the proportion of NCI-H520 cells in cell cycle S-phase increased after treatment of Tanshinone Ⅱ A. Further, Immunoblotting showed the upregulation of cyclin E and CDK inhibitor i〉27, in NCI-H520 cells treated with Tanshinone Ⅱ A. Conclusion： Tanshinone Ⅱ A arrested NCI-H520 cell cycle at S arrest maybe by the upregulation of F27 which inhibits CDK2.

关 键 词：丹参酮ⅡA 非小细胞肺癌细胞 P27 细胞周期 

分 类 号：R734.2[医药卫生—肿瘤]