不同发酵天数对“栓钱菌质”急性毒性及抗炎、镇痛作用的影响  

Effects of Different Fermentation Days on Acute Toxicity and Analgesic,Anti-Inflammatory Activity of Shuanqian Junzhi

作　　者：彭薇[1] 孙钰[1] 潘扬[1] 张弦[1] 蒋亚平[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210029

出　　处：《食品与生物技术学报》2013年第5期541-545,共5页Journal of Food Science and Biotechnology

基　　金：江苏高校优势学科建设工程资助项目(ysxk-2010);南京中医药大学中药学优势学科开放课题(2011ZYX5-001)

摘　　要：通过急性毒性试验、小鼠扭体试验和耳廓肿胀试验,比较7个不同发酵天数(0、7、20、41、48、62、69 d)的"栓钱菌质"的急性毒性及抗炎、镇痛作用,研究了不同发酵天数对其毒性效应和抗炎、镇痛作用的影响。实验结果表明,不同发酵天数"栓钱菌质"毒性下降率(%)大小如下:0 d≈7 d<20 d≈41 d<48 d<62 d<69 d。扭体反应次数大小如下:69 d≈62 d>48 d>41 d≈20 d>0 d>7 d;与空白对照相比,0、7、20、41、48 d者与马钱子生品一样,均明显减少醋酸致小鼠扭体反应次数(P<0.05),表明发酵48 d内基本保留了生品原有的镇痛作用;而62 d和69 d者则此活性消失,且与阳性对照(吲哚美辛)相比有极显著性差异(P<0.01)。耳廓肿胀下降率(%)大小如下:7 d≈20 d>41 d>48 d>0 d>>62 d>69 d;与空白对照相比,0、7 d、20、41、48 d者与生品相同,皆可显著抑制致炎后小鼠的耳廓肿胀率(P<0.05或0.01),表明发酵48 d内抗炎作用没有明显变化。综合分析不同发酵天数"栓钱菌质"毒性和药效的结果,确定其发酵终点应选择在第41～48 d较适宜。To observe the effects of different fermentation days of Shuanqian Junzhi(SQJZ,a Strychnos nux-vomica product fermented by Trametes cinnabarina) on acute toxicity and analgesic,anti-inflammatory activity,seven samples of different fermentation days(0 d,7 d,20 d,41 d,48 d,62 d and 69 d) were applied.The acute toxicity and anti-inflammatory,analgesic activities of SQJZ of different fermentation days were evaluated in animal models.Listed as followed was the acute toxicity of different SQJZ in a descending order:0 d≈7 d <20 d≈41 d <48 d <62 d <69 d.The sustaining twisting reaction times was:69 d≈62 d>48 d>41 d≈20 d>0 d>7 d.According to the control group,the twisting times caused by acetic acid of the crude,0 d,7 d,20 d,41 d,48 d of SQJZ noticeably decreased(P<0.05) just like the crude Strychnos nux-vomica,which indicated the analgesic action of the crude did not change significantly within 48 day-fermentation period.However,62 d and 69 d of SQJZ had no significant analgesic effect and there was a significant difference(P<0.01) in comparison with the positive drug indometacin,which showed a complete loss of the anti-inflammatory effect after 62 day-fermentation.The swelling percent decrease of external ear was:7 d≈20 d>41 d>48 d>0 d>>62 d>69 d.With the control group,0 d,7 d,20 d,41 d and 48 d of SQJZ were able to reduce the swelling of murine external ear caused by croton oil(P<0.05 or 0.01),the same as the crude,indicating that their anti-inflammatory effect did not declined through 48 day-fermentation.Therefore,the feasible period of fermentation of SQJZ is about 41-48 days.

关 键 词：“栓钱菌质” 发酵时间 马钱子 急性毒性 镇痛 抗炎 

分 类 号：R285[医药卫生—中药学]