鱼腥草素钠对铜绿假单胞菌生物被膜的影响  

Effect of Sodium Houttuyfonate on Pseudomonas aeruginosa Biofilm

作　　者：王艳[1] 程惠娟[1] 朱玲玲[1] 孙振新[1] 李露天[1] 

机构地区：[1]安徽中医药大学,安徽合肥230038

出　　处：《食品科学》2013年第11期173-176,共4页Food Science

基　　金：国家自然科学基金面上项目(81173629);安徽中医学院自然科学基金项目(2011zr013B)

摘　　要：目的:研究鱼腥草素钠对铜绿假单胞菌生物被膜生长不同阶段的影响。方法:MTT法检测不同质量浓度鱼腥草素钠对铜绿假单胞菌的抗菌活性及代谢的影响;电镜观察鱼腥草素钠对铜绿假单胞菌生物被膜生长不同阶段的影响;载体滤膜上活菌计数检测鱼腥草素钠对铜绿假单胞菌生物被膜黏附性的影响。结果:鱼腥草素钠对铜绿假单胞菌的最小抑菌质量浓度300μg/mL,减小鱼腥草素钠质量浓度,其抗菌活性亦随之减小;生物被膜形成过程中抑制生物膜50%的质量浓度(SMIC50)在9.37～37.5μg/mL,抑制生物膜80%的质量浓度(SMIC80)在37.5～150μg/mL之间,在此药物质量浓度范围内被膜里的菌量显著减少,与正常对照组相比,差异显著(P<0.05);扫描电镜下观察到药物组细菌呈浮游状态,细菌表面光滑,未见到黏液状物质;鱼腥草素钠药物组载体滤膜上的活菌数明显少于正常对照组的,其中鱼腥草素钠高剂量组在黏附阶段、初步生物被膜形成、成熟生物被膜形成阶段和正常对照组相比差异显著(P<0.05)。结论:鱼腥草素钠作为天然植物鱼腥草的有效成分之一,对铜绿假单胞菌有一定的抗菌活性,能有效抑制铜绿假单胞菌生物被膜的形成。Purpose： To investigate the effect of sodium houttuyfonate on the formation of Pseudomonas aeruginosa biofilm at different stages. Methods： MTT assay was used to detect the antibacterial and metabolic effect of sodium houttuyfonate at different concentrations on Pseudomonas aeruginosa. An electron microscope was used to observe the inhibitory effect of sodium houttuyfonate on the formation of Pseudomonas aeruginosa biofilm at different stages. Viable cell count on filter membrane was used to evaluate the inhibitory effect of sodium houttuyfonate on the adhesion of Pseudomonas aeruginosa biofilm. Results： Minimum inhibitory concentration was 300 μg/mL, and the antibacterial activity of sodium houttuyfonate was positively dependent on its concentration. The concentration necessary for 50% and 80% inhibition of biofilm formation were 9.37--37.5 μg/mL and 37.5-150 μg/mL, respectively, and as a result, biofilm bacterial populations were reduced significantly compared with normal control group （P 〈 0.05）. Scanning electron microscopic （SEM） observation revealed that bacteria in sodium houttuyfonate group were in a planktonic state with smooth surface and no mucus-like substances, and the number of viable cells on cartier in the membrane filter was apparently smallers than that of normal control group. Sodium houttuyfonate at high dose had significant differences from control （P 〈 0.05） in adhesion, initial formation of biofilm and the formation of mature biofilm. Conclusion： As an active ingredient in Houttuynia cordata, sodium houttuyfonate has antibacterial activity against Pseudomonas aeruginosa, and can effectively inhibit the formation of biofilm.

关 键 词：生物被膜 铜绿假单胞菌 鱼腥草素钠 

分 类 号：R378.991[医药卫生—病原生物学]