葡萄糖受体靶向的长循环脂质体的磁共振分子成像研究  

Magnetic resonance molecular imaging of glucose-receptor-targeted long-circulating liposome

作　　者：杨春[1] 张玲[2] 尹燕[1] 陈超[1] 徐凯[1] 郑骏年 

机构地区：[1]徐州医学院附属医院影像科,江苏221002 [2]徐州医学院药学院 [3]江苏省肿瘤生物治疗研究所

出　　处：《中华实验外科杂志》2013年第6期1123-1125,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省自然科学基金资助项目（BK2010178）;江苏省高校自然科学基金资助项目（10KJB320025）;徐州市科技项目（XFllC062）;徐州医学院肿瘤生物治疗重点实验室资助项目（C0803）

摘　　要：目的探讨葡萄糖受体靶向的钆喷酸葡胺（Gd—DTPA）脂质体对裸鼠肾癌移植瘤的靶向性。方法制备聚乙二醇（PEG）修饰的Gd．DTPA脂质体（PEG—Gd．DTPA）及N-棕榈酸葡萄糖胺（NPG）、PEG修饰的Gd—DTPA脂质体（NPG—PEG．Gd—DTPA）。将21只肾癌移植瘤的裸鼠分为3组（n=7），分别经腹腔注射Gd—DTPA、PEG．Gd．DTPA及NPG—PEG．Gd．DTPA后0．5、1．5、2．5、3．5h行磁共振成像（MRI）扫描，计算相对增强值。结果Gd—DTPA组强化峰值出现在0．5h，相对增强值为1．83±0．25；PEG—Gd—DTPA及NPG—PEG—Gd．DTPA组强化峰值均出现在2．5h，相对增强值分别为2．33±0．30、2．87±0．40。3组移植瘤1．5、2．5、3．5h扫描时间点相对增强值比较，差异有统计学意义（P〈0．01），且NPG—PEG—Gd—DTPA组相对增强值最高。结论NPG、PEG修饰Gd—DTPA脂质体能与葡萄糖受体结合，实现MRI靶向成像。Objective To prepare long cycle of gadopentetate dimeglumine （Gd-DTPA） liposome and glucose receptor-targeted long cycle of Gd-DTPA liposome and to study the targeting to the renal ceil car- cinoma. Methods Long cycle of Gd-DTPA liposome and glucose receptor-targeted long cycle of Gd-DTPA li- posome were prepared. Twenty-one male nude mice were divided into 3 groups （7 nude mice in each group） , and T1WI scanning was done at 0. 5, 1.5, 2. 5 and 3.5 h after intraperitoneal injection of Gd-DTPA, long cycle of Gd-DTPA liposome and glucose receptor-targeted long cycle of Gd-DTPA liposome, separately. After the magnetic resonance （MR） scanning, signal intensity was measured and the relative enhanced value （REV） was calculated. Results In vivo MR imaging, the injection of Gd-DTPA resulted in a maximum tumor REV of 1.83±0. 25 at 0. 5 h. The injection of PEG-modified Gd-DTPA liposome and NPG-PEG-modi- fled Gd-DTPA liposome resulted in a maximum tumor REV of 2. 33±0. 30 and 2. 87±0. 40 at 2. 5 h, respectively. There was significant difference in REV among three groups at 1.5, 2. 5, and 3.5 h after injfection （P 〈 0. 01 ）, and the REV of NPG-PEG-modified Gd-DTPA liposome group was the highest. Conclusion Glucose-receptor-targeted long-circulating Gd-DTPA liposome could be combined to tumor cells via glucose receptor and significantly targeted to tumor cells with rich glucose receptors for MR imaging.

关 键 词：肾细胞癌 葡萄糖受体 磁共振成像 脂质体 

分 类 号：R737[医药卫生—肿瘤] R44[医药卫生—临床医学]