胃癌腹膜乳斑转移与趋化因子CCL22及受体CC类趋化因子受体-4的关系  

Contribution of CCL22 and CC chemokine receptor-4 to milky spots peritoneal-metastasis of gastric cancer

作　　者：张伟国[1] 安伟德[1] 苗鑫[2] 邓中慧[1] 

机构地区：[1]大连医科大学附属第一医院普外科,116011 [2]泰山医学院附属医院普外科

出　　处：《中华实验外科杂志》2013年第6期1242-1244,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨趋化因子CCL22及其受体CC类趋化因子受体-4（CCR4）在胃癌腹膜乳斑区转移的作用。方法经Dil标记的胃癌细胞株MFC，小鼠腹腔内注射Dil—MFC细胞悬液0．2ml（CCR4表达阳性细胞数为5X10。个），注射48h后，免疫组织化学方法检测大网膜乳斑区和非乳斑区的CCL22、CCR4表达。615小鼠腹腔注射0．2ml胃癌细胞株MFC悬液（胃癌细胞数为5X10。个），分别于注射后第6、8、10天，采用酶联免疫吸附试验（ELISA）法检测其腹水中CCL22的浓度。结果苏木素．伊红（HE）染色、免疫组织化学检测可见胃癌腹膜转移早期乳斑区域CCL22、CCR4表达均为阳性，而非乳斑区域没有趋化因子CCL22，也没有胃癌细胞转移。小鼠腹水中CCL22浓度在腹腔注射MFC悬液6、8、10d分别为（345．29±1．98）、（378．29±3．87）、（371．57±2．44）ng／L，随着腹水的增加而升高，与空白组比较差异有统计学意义（P〈0．01）。结论趋化因子CCL22及其受体CCR4在胃癌腹膜乳斑转移中起作用。Objective To investigate the function of CCL22 and CC chemokine receptor-4 （CCR4） in the milky spots peritoneal metastasis of gastric cancer. Methods Dil-MFC cells （ CCR4 expression posi- tive, 5×10^6 cells） were injected into mice. The expression of CCR4 and CCL22 in the milky spots region and non-milky sports region was compared by using immunohistochemistry 48 h after injection. The concen- trations of CCL22 in ascitic fluid were determined by using enzyme linked immunosorbent assay （ELISA） at 6th, 8th and 10th day after 0. 2 mL of MFC cells （5×10^6 ceils） was injected into the 615 mice. Results Hematoxylin and eosin （HE） staining and immunohistochemistry indicated that in the early stages of perito- neal metastasis, the CCL22 and CCR4 expression was positive on milky spots, but on the non-milky spots region, there was no CCL22 expression and no metastasis of gastric cancer cells. The concentration of CCL22 in bloody ascites of mice was （345.29 ±1.98 ） , （ 378.29±3.87 ） and （ 371.57 ±2.44） ng/L respectively at 6th, 8th and 10th day after intraperitoneal injection （ P 〈 0. 01 ）. Conclusion CCL22 and CCR4 play an important role in the early peritoneal metastasis.

关 键 词：胃癌 腹膜转移 CCL22 CC类趋化因子受体-4 

分 类 号：R735[医药卫生—肿瘤]