多药耐药相关蛋白4在不同放射敏感性大肠癌细胞中的表达  

Expression of muitidrug resistance-associated proteins 4 protein in colorectal cancer cell lines with different radiosensitivity

作　　者：柴瑞[1] 于志奇[1] 傅传刚[1] 王颢[1] 左志贵[1] 张畅[1] 

机构地区：[1]浙江省人民医院肛肠外科,杭州310014

出　　处：《中华实验外科杂志》2013年第6期1255-1258,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30973460）

摘　　要：目的通过对大肠癌放射敏感细胞株与抵抗细胞株的筛选，观察多药耐药相关蛋白4（MRP4）在2株细胞中的表达，探讨MRP4蛋白表达与放射敏感性的关系。方法用不同放射剂量（0、2、4、6、8Gy）X射线照射大肠癌细胞株LOVO、DLD-1、HT-29、HCT116、RKO并从中筛选出放射敏感和抵抗细胞株，免疫组织化学方法检测MRP4蛋白在这2株细胞中的表达差异。结果放射敏感细胞株LOVO与放射抵抗细胞株RKO在射线干预后生长抑制曲线差异有统计学意义（P〈0．01）；MRP4蛋白在RKO与LOVO中的低表达率分别为71％、17％和29％、83％，差异有统计学意义（P〈0．01）。结论MRP4蛋白在放射敏感细胞株LOVO中低表达，而在放射耐受细胞株RKO中高表达，表明MRP4蛋白表达不仅和化疗抵抗相关，还与肿瘤的放射抵抗相关。Objective To screen the radiation-resistant and radiation-sensitive cell strains from 5 colorectal cancer cell lines and observe the muitidrug resistance-associated proteins 4 （MRP4） protein ex- pression in two cell lines, and explore the relashionship between MRP4 protein expression and radiation sensitivity. Methods Different radiation doses （0, 2, 4, 6 and 8 Gy） were used to irradiate colonrectal cancer cell lines LOVO, DLD-1, HT-29, HCT116 and RKO, and radiation sensitive and radiation resistant cell lines were screened. Immunohistochemistry was used to detect MRP4 protein expression in these two cell lines. Results Radiation sensitive and radiation resistant cell lines were LOVO and RKO separately. Their growth inhibition curve after the intervention was significantly different （P 〈 0. 01 ）. For MRP4 pro- tein expression in the radiation resistant RKO and sensitive LOVO cell lines, high expression and low ex- pression rate was 71% and 17% , and 29% and 83% separately with the difference being significant （P 〈 0.01 ）. Conclusion The low-expression of MRP4 protein in radiation-sensitive cell line LOVO and the high-expression of MRP4 protein in RKO radiation-resistant cell line reveals that MRP4 protein expression is not only related to chemotherapy resistance, but also radiotherapy resistance.

关 键 词：大肠癌 放射敏感性 多药耐药相关蛋白4 

分 类 号：R735[医药卫生—肿瘤]