荔枝核提取物对D-半乳糖诱致小鼠学习记忆障碍的影响及其机制  

Effect of Litchi Seed Aqueous Extracts on Learning and Memory Obstacles Induced by D-galactose in Mice and Its Mechanism

作　　者：叶红梅[1] 钟春燕[2] 黄敏贤[1] 王晨羽[3] 冯欣[3] 陈晓韵[3] 吕俊华[2] 

机构地区：[1]广州市中医医院药剂科,广东广州510130 [2]暨南大学药学院药理教研室,广东广州510632 [3]暨南大学医学院,广东广州510632

出　　处：《中药材》2013年第3期438-440,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:探讨荔枝核提取物对学习记忆障碍的影响及其机制。方法:50只昆明种小鼠随机分为空白对照组、模型组、石杉碱甲组(0.4 mg/kg)及荔枝核提取物低(15 g/kg)、高(60 g/kg)剂量组。上述动物除空白对照组外,均皮下(sc)注射D-半乳糖(500 mg/kg)8 w,并于第5周起分别灌胃相应药物。采用水迷宫和自主活动仪测试小鼠学习和记忆能力,并测定血清糖基化终末产物(AGEs)及脑组织一氧化氮(NO)、乙酰胆碱(Ach)的含量和一氧化氮合酶(NOS)、乙酰胆碱酯酶(AchE)活性。结果:荔枝核提取物能显著改善模型小鼠学习和记忆能力,减少血清糖基化产物(AGEs)含量,同时降低脑组织NO含量和NOS活性,对脑组织Ach含量、AchE的活性无明显影响。结论:荔枝核提取物具有改善D-半乳糖诱致小鼠学习记忆障碍的作用,其机制可能与抑制过多的AGEs和NO等对脑细胞损伤作用有关。Objective：To investigate the improving effect of Litchi Seed Aqueous Extracts on learning and memory obstacles model and its mechanism.Methods：The learning and memory obstacles model was incluced by subcutoneous injection of D-galactose（500 mg/kg）for 8 weeks.The model group and treatment groups were given huperzine A（0.4 mg/kg）and Litchi Seed Aqueous Extracts（15,60 g/kg）respectively for 4 weeks by ig at the 5th week.After huperzine A and Litchi Seed Aqueous Extracts treatment for 4 weeks,water maze test was used to determine the ability of mice＇s spatial learning and memory.The contents of advanced glycation end products（AGEs） in serum,the content of nitric oxide（NO） and acetylcholine（Ach）,the activity of nitric oxide synthase（NOS） and acetylcholinesterase（AchE） in the brain tissue were detected.Results：Litchi Seed Aqueous Extracts significantly ameliorated the learning and memory ability in mice,decreased the level of AGEs in serum,and reduced the content of NO and activity of NOS in brain tissues.No significantly influence was observed for the Ach and Ach-E in brain tissues.Conclusion：Litchi Seed Aqueous Extracts possesses improving the learning and memory effects on the model mice induced by D-galactose,which may be related to inhibiting too much AGEs and NO formation and reducing damage in the brain cells.

关 键 词：荔枝核提取物 D-半乳糖 学习记忆 糖基化产物 一氧化氮 

分 类 号：R285.5[医药卫生—中药学]