假眼小绿叶蝉地理种群的线粒体DNA 16S rRNA基因序列分析及遗传分化研究  

Sequence analysis of the mtDNA gene and genetic differentiation in geographic populations of Empoasca vitis

作　　者：李乐[1] 付建玉[1] 肖强[1] 

机构地区：[1]中国农业科学院茶叶研究所,杭州310008

出　　处：《应用昆虫学报》2013年第3期675-685,共11页Chinese Journal of Applied Entomology

基　　金：科技基础性工作专项(2008FY210500);国家科技支撑计划课题(2011BAD01B02)

摘　　要：测定了12个不同地理种群的茶树害虫假眼小绿叶蝉Empoasca vitis(Gothe)和1个外群共103个个体的线粒体DNA 16S rRNA基因部分序列,比较其同源性,计算核苷酸组成,并构建单倍型进化关系图。结果表明:在获得的假眼小绿叶蝉536 bp的序列中A+T含量占76.8%,其中56个核苷酸位点存在变异;100个个体共检测出56种单倍型,单倍型多样性指数(H)为0.971,核苷酸多样性指数(Pi)0.006;12个种群间的平均基因流(Nm)为1.64。总群体的固定系数Fst为0.026;AMOVA分子方差分析结果表明假眼小绿叶蝉的遗传分化主要来自于种群内部(97.4%)。各地理种群的遗传距离与地理分布不具有直接对应的关系。根据构建的单倍型进化关系网,各地理种群的单倍型呈现一种混杂的分布格局,未显示出与地理分布的一致性。Mitochondrial DNA 16S rRNA gene sequences were sequenced in 103 individuals of Empoasca vitis （Gothe） from 12 different geographical populations, including an outgroup species （Erythroneura sudra D.） . We compared the homologous sequences of all the individuals, and then calculated nucleotide composition. The median-joining network was constructed by using Network 4500. Through the sequential analyses, we observed that the value of A＋T was about 76.8% and 56 nucleotide sites were substituted in the 536bp fragment of Empoasca vitis （Gothe）. 56 haplotypes were detected in 100 individuals. Haplotype diversity index （H） and mean nucleotide diversity index （Pi） reached 0.971 and 0.006 respectively. The gene flow （Nm） among the 12 populations was 1.64. The overall Fst value was 0.026. Molecular variance （AMOVA） analysis indicated that a high proportion of the total genetic variance was attributable to variations with in populations （97.4%）. The median-joining network of the 56 haplotypes did not distinguish distinctly into 12-location groupings of haplotypes and did not correspond to every geographic group of Empoasca vitis （Gothe）.

关 键 词：茶树 假眼小绿叶蝉 地理种群 线粒体16S rRNA 遗传分化 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]