中华鳖日本品系体内氟苯尼考药物代谢动力学研究  

Pharmacokinetics of Florfenicol in Pelodiscus sinensis Japanese Strain

作　　者：张海琪[1,2] 林静[2] 黎飞[2] 杜伟[2] 王扬[2] 

机构地区：[1]浙江大学生命科学学院,杭州310058 [2]浙江省水产质量检测中心,杭州310012

出　　处：《动物学杂志》2013年第3期465-473,共9页Chinese Journal of Zoology

基　　金：浙江省科技厅重大攻关项目(No.2009C12075);浙江省重点标准化研究项目(No.2008-6)

摘　　要：应用液相色谱-串联质谱技术研究了氟苯尼考在中华鳖日本品系(Pelodiscus sinensis Japanesestrain)体内的残留代谢规律。在(25.0±2.0)℃水温下,150只体重为(250±50)g健康中华鳖日本品系连续7 d投喂含30 mg/kg氟苯尼考的饲料,分别于最后一次给药后1 h、2 h、4 h、8 h、16 h、24 h、48 h、72 h、96 h、120 h、144 h、168 h、240 h、360 h采集肝、血液、肌肉和肾样品。样品中氟苯尼考的残留量采用乙酸乙酯提取,正己烷净化,电喷雾负离子多反应监测模式下进行测定,内标法定量,并利用3P97药动学软件进行数据分析。结果表明,氟苯尼考在中华鳖日本品系血液、肌肉、肝和肾中达峰浓度分别为167μg/kg、188μg/kg、67.15μg/kg和85.71μg/kg,达峰时间分别为4 h、8 h、8 h和8 h,消除半衰期分别为14.9 h、9.4 h、29.5 h和13.0 h,药动学方程分别为:C血液=210.332e-0.070 6 t+938.161e-0.046 5 t,C肌肉=5 642.635e-0.077 1 t+5 765.891e-0.074 4 t,C肝=111.596e-0.036 0 t+4.339e-0.023 5 t和C肾=176.509e-0.065 4 t+615.697e-0.053 6 t。说明氟苯尼考在中华鳖日本品系体内残留代谢较快,建议休药期为15 d。Pharmacokinetics of florfenicol were studied in tissues from Pelodiscus sinensis Japanese strain using high performance liquid chromatography-tandem mass spectrometry. One hundred fifty healthy soft-shell turtles with the weight of 250 ± 50 g were adnubustered florfenicol at a dosage of 30 mg/kg for 7 consecutive days under the temperature of 25.0±2. 0℃. After the last feeding, tissues including blood, muscle, liver and kidney were collected at 1 h, 2 h, 4 h, 8 h, 16 h,24 h, 48 h, 72 h, 96 h, 120 h, 144 h, 168 h, 240 h and 360 h, respectively. Florfenieols in different tissues were extracted with ethyl acetate, cleaned by hexane, and detected by HPLC-MS/MS with negative electrospray ionization under multiple reaction monitoring. Quantitative analysis was used by internal standard method. Results showed that： The peak times of concentrations of florfenicol in plasma, muscle, liver and kidney were 4 h, 8 h, 8 h and 8 h, with the maximum content of 167μg,/kg, 188 μg,/kg, 67. 15 μg/kg and 85.71 μg/kg, respectively. The elimination half life wasl4.9 h, 9.4 h, 29.5 h and 13.0 h.The concentration-time curves of florfenicol in blood, liver, muscle and kidney were： Cblood=210.332e^（-0.0706）＋938.161e^（-0.0465）, Cmescle=5642.635e^（-0.0771）＋5765.891e^（-0.0744t）, Cliver=111.596e^（-0.0360t）＋4.339e^（-0.0235t） and Ckidney=176.509e^（-0.0654t）＋615.697e^（-0.0536t）, respectively. Florfenicol was absorbed and eliminated fast in P. sinensis Japanese strain. The withdraw period should not be less than 15 d under this experiment condition.

关 键 词：中华鳖日本品系 氟苯尼考 药动学 液相色谱-串联质谱 

分 类 号：S948[农业科学—水产养殖]