LBH589或联合多烯紫杉醇对人卵巢癌OVCAR-3细胞增殖和凋亡的研究  

Effects of LBH589 alone or in combination with DTX on the proliferation and apoptosis of the human ovarian cancer line OVCAR-3

作　　者：晁宏图[1,2] 邓君丽[1,2] 马一鸣[1,2] 王莉[1,2] 

机构地区：[1]河南省肿瘤医院妇瘤科 [2]郑州大学附属肿瘤医院妇瘤科,郑州市450003

出　　处：《中国肿瘤临床》2013年第10期567-570,共4页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨新型组蛋白去乙酰化酶抑制剂LBH589或联合多烯紫杉醇(DTX)对人卵巢癌OVCAR-3细胞增殖和凋亡的影响。方法:采用不同浓度LBH589、DTX或两者联合处理OVCAR-3细胞,用四甲基偶氮唑蓝(MTT)法检测细胞增殖活力,台盼兰、吖啶橙溴化乙啶(AO/EB)双染色法检测细胞凋亡;Western blot检测聚腺苷二磷酸核糖聚合酶(PARP)、caspase-3、caspase-7、bcl-2、bax蛋白水平。结果:LBH589、DTX均能抑制OVCAR-3细胞增殖,诱导凋亡,小剂量LBH589联合DTX作用更强。经Calcusyn软件分析证明两者联合具有明显的协同作用。Western blot检测发现caspsae-3、PARP-85kD剪切蛋白增加,bax表达增加,bcl-2表达减少。caspase-7无明显变化。结论:LBH589、DTX能抑制OVCAR-3细胞增殖,诱导凋亡,两者联合有明显的协同作用。Objective： This work aimed to investigate the effects of LBH589, a novel histone deacetylase （HDAC） inhibitor, alone or in combination with docetaxel （DTX） on the proliferation and apoptosis of the human ovarian cancer cell line OVCAR-3. Methods： OVCAR-3 cells were treated with LBH589, DTX, or a combination of both at various concentrations. The proliferation confirmed by Calcusyn software analysis. OVCAR-3 cells apparently increased in number in the combination therapy than in either LBH589 or DTX therapy alone. Western blot analysis showed that the expression of cleaved PARP-85KD, caspase-3, and bax increased, whereas bcl-2 was downregulated. Conclusion： LBH589 and DTX in vitro can significantly inhibit the proliferation of human ovarian cancer cell line OVCAR-3 and induce cell apoptosis of these cells. Combined LBH589 and DTX treatment against drug-resistant OVCAR-3 cells resulted in a synergistic effect of increased sensitivity to chemotherapy.

关 键 词：LBH589 卵巢癌 多烯学杉醇 联合治疗 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]