布-加综合征大鼠动物模型的建立  

Establishment of Budd-Chiari Syndrome Model in Rat

作　　者：李健[1] 马秀现[1] 孙玉岭[1] 许培钦[1] 赵云峰[1] 

机构地区：[1]郑州大学第一附属医院肝胆外科,河南郑州450052

出　　处：《中国普外基础与临床杂志》2013年第5期477-480,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金项目(项目编号:81100304)~~

摘　　要：目的探索采用下腔静脉缩窄术建立布-加综合征大鼠动物模型的可行性。方法将50只SD大鼠采用完全随机法随机分为实验组和假手术组,全麻开腹,分离肝上下腔静脉。实验组借鉴缩窄门静脉主干法,丝线环绕结扎肝后下腔静脉,使下腔静脉横截面积缩窄约80%。2组分别于术后不同时间点(第1、4、8、12周)行腹部彩超、肝功能、血常规及肝脏病理学检查。结果实验组在第4周时均出现肝后下腔静脉及主肝静脉梗阻,淤血性肝硬变,腹水,肝脾肿大,门静脉扩张,肝实质内交通支开放,而假手术组正常。模型建立后第4周时,实验组大鼠肝功能中ALB及血常规中WBC、PLT、RBC、HGB较假手术组明显降低(P<0.05),ALT、AST、AKP、TBIL、DBIL、TBA较假手术组明显升高(P<0.05)。结论下腔静脉缩窄术可成功建立布-加综合征大鼠动物模型。Objective To explore the feasibility of the Budd-Chiari syndrome model establishment in rat by using the inferior vena cava coarctation.Methods Fifty SD rats were randomly divided into experimental group and sham operation group,the laparotomy was performed after general anesthesia by intraperitoneal injection,and dissociated the inferior vena cava.In the experimental group,the vena cava was tightly ligated with silk thread according to partial portal vein coarctation,enclosing 23 G L-style blunt needle in the ligature to prevent complete obliteration.The diameter of the vena cava was set to about 80% of its normal size after removing the 23 G L-style blunt needle.The abdominal Doppler,liver function,blood routine examination,and liver biopsy were tested at different time（on week 1,4,8,and 12） after operation.Results The signs of inferior vena cava and primary hepatic venous obstruction,liver congestion and cirrhosis,ascites,hepatosplenomegaly,portal vein extension,and collateral patency occurred on week 4 in the experimental group.The levels of AST,ALT,AKP,TBIL,DBIL,and TBA in the experimental group were significantly higher than those in the sham operation group（P〈0.05）,and the WBC,PLT,RBC,HGB,and ALB in the experimental group was significantly lower than those in the sham operation group（P〈0.05）.Conclusion The inferior vena cava coarctation can be successfully used to establish a rat model of Budd-Chiari syndrome.

关 键 词：布-加综合征 动物模型 病理生理 

分 类 号：R-332[医药卫生]