胃癌原发灶中上皮间质转化相关因子和CD133的表达及其与临床病理特征和预后的关系  

Epithelial-Mesenchymal Transition Related Factors and CD133 Protein Expressions in Primary Lesion of Gastric Cancer and Its Relationship with Clinicopathologic Features and Prognosis

作　　者：蔡成[1] 俞继卫[1] 吴巨钢[1] 陆瑞祺[1] 倪晓春[1] 王守练[1] 姜波健[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院普外一科,上海201900

出　　处：《中国普外基础与临床杂志》2013年第5期492-498,共7页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金资助(项目编号:81101850);上海市科委基金资助(项目编号:09411962300);上海市卫生局基金资助(项目编号:2010018)~~

摘　　要：目的研究上皮间质转化(EMT)相关因子Snail、E-cadherin、N-cadherin与胃癌患者临床病理特征、预后及胃癌肿瘤起始细胞表面标志物CD133表达的关系。方法利用Western blot方法检测50例胃癌及癌旁正常胃黏膜组织中EMT相关因子及CD133蛋白的定位及定量表达,分析EMT相关因子及CD133蛋白表达与胃癌患者的临床病理学指标的关系,Spearman等级相关分析EMT相关因子和CD133表达的关系,Kaplan-Meier方法分析EMT相关因子及CD133表达与胃癌患者生存的关系。结果①胃癌组织中Snail、N-cadherin及CD133蛋白表达相对灰度值明显高于其在癌旁正常胃黏膜组织中的表达(Snail:0.599±0.114比0.259±0.108,P=0.020;N-cadherin:0.754±0.154比0.329±0.134,P=0.001;CD133:0.635±0.119比0.485±0.116,P=0.029),E-cadherin蛋白表达相对灰度值明显低于其在癌旁正常胃黏膜组织中的表达(0.378±0.123比0.752±0.156,P=0.003)。②Snail蛋白、N-cadherin蛋白表达平均相对灰度值在有血管浸润、淋巴管浸润、N3淋巴结转移及肿瘤直径≥5 cm和Ⅲ+Ⅳ期胃癌患者中的表达明显高于无血管浸润、淋巴管浸润、N0～N2淋巴结转移及肿瘤直径<5 cm和Ⅰ+Ⅱ期的胃癌患者(P<0.05),而E-cadherin蛋白表达平均相对灰度值在有血管浸润、淋巴管浸润、N3淋巴结转移及Ⅲ+Ⅳ期胃癌患者中的表达显著低于无血管浸润、淋巴管浸润、N0～N2淋巴结转移及Ⅰ+Ⅱ期的胃癌患者(P<0.05),CD133蛋白表达平均相对灰度值在有淋巴管浸润、N3淋巴结转移、肿瘤直径≥5 cm和Ⅲ+Ⅳ期胃癌患者中的表达显著高于无淋巴管浸润、N0～N2淋巴结转移、肿瘤直径<5 cm和Ⅰ+Ⅱ期的胃癌患者(P<0.05)。③Snail、N-cadherin蛋白表达与CD133蛋白表达分别均呈正相关(rs=0.278,P=0.048;rs=0.406,P=0.003),而E-cadherin蛋白表达与CD133蛋白表达呈负相关(rs=-0.504,P=0.000)。④Snail、N-cadherin及CD133蛋白低表达组的生存时间明显长于其高表达者(P<0.05),Objective To investigate the prognostic value of epithelial-mesenchymal transition（EMT） related proteins（Snail,E-cadherin,and N-cadherin） in gastric cancer and its relationship with tumor initiating cells（TICs） marker（CD133）.Methods The expressions of EMT-related proteins and CD133 protein in the gastric cancer tissues and normal gastric mucosa tissues adjacent to gastric cancer were detected by Western blot method.The relations between the expressions of EMT-related factors proteins and CD133 protein and the clinicopathologic characters were analyzed.The correlations between EMT-related factors and CD133 were analyzed by Spearman.The correlations between EMT-related factors expressions and CD133 expression and survival were analyzed by Kaplan-Meier method and Log-rank test.Results ① The protein expression levels of Snail,N-cadherin,and CD133 in the gastric cancer tissues were significantly higher than those in the normal gastric mucosa tissues adjacent to gastric cancer（Snail：0.599± 0.114 versus 0.259±0.108,P=0.020;N-cadherin：0.754±0.154 versus 0.329±0.134,P=0.001;CD133：0.635± 0.119 versus 0.485±0.116,P=0.029）,while the protein expression level of E-cadherin was lower than that in the normal gastric mucosa tissues adjacent to gastric cancer（0.378±0.123 versus 0.752±0.156,P=0.003）.② The expression levels of Snail and N-cadherin in the gastric cancer patients with vascular invasion,lymphatic vessel invasion,N3 lymph node metastasis,diameter more than 5 cm,and Ⅲ＋Ⅳ staging were significantly higher than those in the patients without vascular invasion,lymphatic vessel invasion,N0-N2 lymph node metastasis,diameter less than 5 cm,andⅠ＋Ⅱ staging（P〈0.05）,while E-cadherin protein expression was lower than that in the patients without vascular invasion,lymphatic vessel invasion,N0？-？N2 lymph nodes metastasis,andⅠ＋Ⅱstaging（P〈0.05）.The expression levels of CD133 in the gastric cancer patients with lymphatic vessel invasion,diameter more than 5 cm,and

关 键 词：胃 癌 新生物 上皮间质转化 CD133 预后 Westernblot方法 

分 类 号：R735.2[医药卫生—肿瘤]