乳腺癌干细胞激素受体与HER-2表达生物学意义的分析  

Expression and significance of ER,PR,HER-2 in breast cancer stem cells

作　　者：桑晶[1] 孙勇[2] 韩玉贞[3] 

机构地区：[1]泰安市中心医院病理科,山东泰安271000 [2]泰安市中心医院儿内科,山东泰安271000 [3]滨州医学院病理教研室,山东滨州256603

出　　处：《中华肿瘤防治杂志》2013年第12期926-929,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：山东省医药卫生科技计划(2009HZ001)

摘　　要：目的:探讨雌激素受体(ER)、孕激素受体(PR)和人类表皮生长因子受体2(HER-2)在乳腺癌干细胞(BCSCs)和其分化细胞中的表达及意义。方法:选取乳腺浸润性导管癌新鲜标本30例,采用机械分离法将乳腺癌组织块制备成单细胞悬液,通过免疫磁珠两步法从中分离出BCSCs(CD44+CD24-/low细胞)和乳腺癌分化细胞(CD24+细胞和CD44-CD24-细胞),应用免疫细胞化学Envision二步法检测两组细胞ER、PR和HER-2的表达情况。结果:BCSCs ER-表达率为83.3%(25/30),分化细胞ER-表达率为53.3%(16/30),两者差异有统计学意义,P=0.025;BCSCs PR-表达率为76.7%(23/30),分化细胞PR-表达率为23.3%(7/30),两者差异有统计学意义,P=0.000;BCSCs HER-2-表达率为83.3%(25/30),分化细胞HER-2-表达率为53.3%(16/30),两者差异有统计学意义,P=0.025;BCSCs和分化细胞表型构成不同(P=0.000),BCSCs以ER-、PR-及HER-2-表型为主,占66.7%(20/30),乳腺癌分化细胞以ER和(或)PR+、HER-2-表型为主,占43.3%(13/30)。结论:BCSCs ER、PR和HER-2均可呈现阳性或阴性表达,但以阴性表达为主,随着BCSCs的分化,其阳性表达率明显升高。BCSCs的表型以ER-、PR-及HER-2-为主,这可能是部分患者内分泌治疗失败的主要原因。OBJECTIVE：To separate breast cancer stem cells（BCSCs） from primary human breast cancer tissue and explore the expression and significance of ER,PR, HER-2,in BCSCs. METHODS： Thirty fresh specimens of breast inva- sive ductal carcinoma were processed into single cell suspensions by mechanical separation, then BCSCs （CD44＋/ CD24-/loW cells） and differentiated cells （CD24＋ and CD44-/CD24- cells） were separated from them by immunomagnetic sorting. Immunocytochemical technique was employed to detect the expression of ER,PR, HER-2 in two groups. RE- SULTS.. The negative rates of ER in BCSCs were 83.3 % （25/30）, in differentiated cells were 53.3 % （16/30）, the differ- ences were significant （P=0. 025） ; The negative rates of PR in BCSCs were 76.7 % （23/30）, in differentiated cells were 23.3%（7/30）, the differences were significant （P=0. 000）; The negative rates of HER-2 in BCSCs were 83. 3%（25/30） ,in differentiated cells were 53.3 % （16/30）, the differences were significant （P=0. 025）. The phenotype was dif- ferent too （P= 0. 000）. ER--, PR-- and HER-2- were the predominant phenotypes in BCSCs, accounting for about 66.7 % （20/30）. ER and/or PR＋, HER-2-- were the predominant phenotype in differentiated cells, accounting for about 43.3 % （13/30）. CONCLUSIONS： The expression of ER,PR and HER-2 in BCSCs can be either positive or negative, yet the majority of them are negative; With the differentiation of BCSCs,the positive rates will be higher. ER--,PR-- and HER-2-- are the predominant phenotype in breast cancer stem cells,which may relate to the failure of endocrine therapy.

关 键 词：乳腺肿瘤 干细胞 免疫磁珠分选 雌激素受体 孕激素受体 人类表皮生长因子受体2 

分 类 号：R737.9[医药卫生—肿瘤]