不同中医临床证型下行型鼻咽癌组织细胞核蛋白质差异表达的初步研究  

Different nuclear protein expression in downward progressive nasopharyngeal carcinoma

作　　者：何迎春[1,2] 刘宇勤[3] 廖端芳[4] 戴娜[5] 何兰[6] 范婧莹[1] 田道法[7] 

机构地区：[1]湖南中医药大学中西医结合学院,湖南长沙410208 [2]南华大学医学院,湖南衡阳421001 [3]怀化市中医院,湖南怀化418000 [4]湖南中医药大学干细胞中药调控与应用实验室,湖南长沙410208 [5]湖南中医药大学第二中医临床学院,湖南长沙410208 [6]湖南中医药大学中医学院,湖南长沙410208 [7]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《癌变．畸变．突变》2013年第3期183-189,共7页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(81273988;81072904);中国博士后基金(2011M501277);湖南省外国专家局引智项目(湘财社指[2011]民40号;湘教通[2012]488号);湖南省中医五官科学重点学科项目

摘　　要：目的:研究下行型鼻咽癌不同中医临床证型患者原发病灶组织细胞核差异表达蛋白质,为中医证型分类的科学性提供依据。方法:采用双向凝胶电泳及基质辅助激光解吸电离-飞行时间质谱法(MALDI-TOF-MS)对下行型鼻咽癌火毒困结型、气阴两虚型和气血凝结型患者的鼻咽癌组织细胞核蛋白进行分离和表达谱研究,利用生物信息学方法鉴定差异蛋白质。结果:与下行型鼻咽癌气阴两虚型细胞核蛋白点相比,气血凝结型有28个蛋白点表达上调、12个蛋白点表达下调,火毒困结型有9个点表达上调,19个蛋白点表达下调;与下行型鼻咽癌气血凝结型细胞核蛋白点相比,火毒困结型有25个蛋白点表达上调、18个蛋白点表达下调。MALDI-TOF-MS成功鉴定出3个差异蛋白质,来源于气血凝结型鼻咽癌患者的瘤组织细胞不均一性核糖核蛋白H(heterogeneous nuclear ribonucleoprotein H,hnRNPH)和微管蛋白β链1(tubulinbeta-1chain,TUB1)表达上调,来源于火毒困结型鼻咽癌患者瘤细胞的核蛋白Ⅵ型胶原蛋白α2链(type Ⅵ collagen alpha 2 chain,COLⅥA2)前体表达下调。结论:下行型气血凝结型鼻咽癌患者的瘤细胞核蛋白hnRNPH和TUB1表达上调,下行型火毒困结型鼻咽癌患者的瘤细胞核蛋白中COLⅥA2前体表达下调。OBJECTIVE： To study the different nuclear protein expression in primary focus of downward progressive type of nasopharyngeal carcinoma in patients with different clinical patterns according to TCM classification. METHODS：Nuclear proteins profiles in fire-toxin stagnation type,Qi-yin deficiency type and Qi-blood coagulation type were measured by two-dimensional gel electrophoresis and matrix assisted laser. RESULTS：Compared with the expression of nuclear protein spots from patients with Qi-yin deficiency type,there were 28 up-regulated protein spots and 12 down-regulated protein spots from patients with Qi-blood coagulation type. We also found 9 up-regulated protein spots and 19 down-regulated protein spots from patients with fire-toxin stagnation type. Compared with the expression of nuclear protein spots from patients with Qi-blood coagulation type,there were 25 up-regulated protein spots and 18 down-regulated protein spots from patients with fire-toxin stagnation type. Three differential proteins were identified successfully by MALDI-TOF-MS. Up-regulated proteins in one spot were heterogeneous nuclear ribonucleoprotein H （hnRNP H） and tubulin beta-1 chain （TUB1） from patients with Qi-blood coagulation type and the other down-regulated protein spot was type VI collagen alpha 2 chain （COLⅥA2） precursor. CONCLUSION：Heterogeneous nuclear ribonucleoprotein H （hnRNP H） and tubulin beta-1 chain （TUB1） in nasopharyngeal carcinoma tissues from patients with Qi-blood coagulation type were up-regulated,type VI collagen alpha 2 chain precursor in those tissues from patients with fire-toxin stagnation type was down-regulated.

关 键 词：下行型鼻咽癌 中医证型 核蛋白质 差异表达 

分 类 号：R739.6[医药卫生—肿瘤]