聚丁二酸丁二醇酯浸提液对人外周血单核细胞的体外遗传毒性  

In vitro genotoxicity of biodegradable poly (butylene succinate) in human peripheral blood mononuclear cells

作　　者：段长恩[1] 公兵[1] 杨秀滨[1] 

机构地区：[1]北京协和医学院中国医学科学院,阜外心血管病医院成人心脏外科,北京100037

出　　处：《癌变．畸变．突变》2013年第3期215-217,221,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(30870620);北京市自然科学基金(2112045)

摘　　要：目的:研究生物可降解材料聚丁二酸丁二醇酯[poly(butylene succinate),PBS]浸提液对人外周血单核细胞(peripheral blood mononuclear cells,PBMCs)的体外遗传毒性。方法:参照GB/T16886-2005标准制备PBS浸提液。采用MTT试验评价不同浓度(200、100、50、25、12.5、6.25、3.125mg/mL)PBS浸提液对PBMCs的细胞毒性;并进行染色体畸变试验和微核试验评价其体外遗传毒性。同时设溶剂RPMI-1640和医用级左旋聚乳酸(L-polylactide,L-PLA)对照组。结果:PBS浸提液对PBMCs的细胞毒性与溶剂对照和L-PLA比较差异均无统计学意义(P>0.05);200mg/mLPBS浸提液在代谢活化和非代谢活化条件下与PBMCs共培养48h后染色体畸变均为阴性;200mg/mLPBS浸提液诱发的微核细胞数与溶剂对照组和L-PLA组相比差异均无统计学意义(P>0.05)。结论:在本试验条件下,生物可降解材料PBS浸提液在体外对PBMCs无遗传毒性,其影响与医用级L-PLA相当。OBJECTIVE： To evaluate the in vitro genotoxicity of PBS [poly（butylene succinate）] in human peripheral blood mononuclear cells. METHODS：The cytotoxicity in PBMCs of PBS including different concentrations （200,100,50,25,12.5,6.25,3.125 mg/mL） was assessed by MTT and the genotoxicity of extracts was evaluated by chromosomal aberration test and micronucleus test. Meanwhile RPMI-1640 and medical biodegradable L-PLA （L-polylactide） were used as control groups. RESULTS：There was little toxicity in the extracts of PBS and no IC50 was valued. There were no cells with chromosomal aberration after co-culture with 200 mg/mL PBS for 48 h under metabolic and non-metabolic activation. The results of micronucleus test compared with the RPMI-1640 and L-PLA groups were not significantly different （P0.05）. CONCLUSION：PBS showed less genotoxicity in peripheral blood mononuclear cells under this experimental condition. It may be a promising medical biodegradable polymer because of excellent biocompatibility.

关 键 词：聚丁二酸丁二醇酯 遗传毒性 染色体畸变 微核试验 

分 类 号：R994.1[医药卫生—毒理学]