DNS法测定红松松塔多糖含量的研究  

Determination of Polysaccharides in Pinus koraiensisCorns with DNS Method

作　　者：冯雪[1] 张曜武[1] 曹炳星[1] 王薇薇[1] 

机构地区：[1]青岛科技大学化工学院,山东青岛266042

出　　处：《中国林副特产》2013年第3期32-35,共4页Forest By-product and Speciality in China

摘　　要：为建立红松松塔多糖含量测定方法。应用DNS法分别测定还原糖及水解后总糖的含量,计算得出多糖含量。结果表明,以葡萄糖为对照品,此方法在20～67mg/L浓度范围内线性关系良好,回归方程A=18.7C-0.2232(R2=0.9993),平均加样回收率99.47%,RSD=1.68%。此法稳定性、重现性、精密度等均在规定范围内,过程简单,操作简便,可快速得出结果。Objective To establish a method for the determination of polysaccharides in Pinus ko- raiensis corns. Methods Determine the content of reducing sugar and total sugar by DNS Meth- od. Results Contrast to glucose,the linear range was 20-67mg/L （R2=0. 999 3）,the average re- covery was 99.47%, RSD= 1.68%. Conclusion The method is sensitive,steady with good repeat- ability.

关 键 词：红松 多糖 含量测定 DNS法 

分 类 号：TS201.3[轻工技术与工程—食品科学]