CK5/6、p63、TTF-1和napsin-A在低分化非小细胞肺癌中区分鳞癌和腺癌的意义  

CK5 /6,p63,TTF-1 and napsin-A immunostaining for distinguishing of poorly differentiated pulmonary non-small carcinomas between adenocarcinoma and squamous cell carcinoma

作　　者：潘黎明[1] 周涛[2] 

机构地区：[1]浙江省玉环县人民医院,玉环317600 [2]浙江省台州医院

出　　处：《浙江创伤外科》2013年第3期305-307,共3页Zhejiang Journal of Traumatic Surgery

摘　　要：目的探讨细胞角蛋白5/6(cytokeratin5/6,CK5/6)、p63、甲状腺转录因子1(thyroid transcription factor-1,TTF-1)和napsin-A联合应用在低分化非小细胞肺癌中区分鳞癌和腺癌的效果。方法 65例低分化非小细胞肺癌,其中腺癌30例、鳞癌35例,免疫组织化学法检测它们的CK5/6,p63,TTF-1和napsin-A的表达,分析它们对低分化非小细胞肺癌中肺鳞癌和腺癌诊断的敏感性和特异性。结果 CK5/6和p63在鳞癌中的阳性率分别是94.3%和100%,而在腺癌中的阳性率分别是0%和6.7%;TTF-1和napsin-A在腺癌中的阳性率分别是96.7%和100%,而在鳞癌中的阳性率是2.9%和0%(全部P<0.05)。CK5/6,p63检测对低分化非小细胞中鳞癌诊断的敏感性和特异性分别为94.3%、100%和100%、93.3%;而TTF-1,napsin-A检测对腺癌诊断的敏感性和特异性分别为96.7%、97.1%和100%、100%。结论 CK5/6,p63,TTF-1和napins-A联合检测是低分化非小细胞肺癌中区分鳞癌和腺癌的较好免疫组化模式。Objective To explore the value of cytokeratin 5/6 （CK5/6）, p63, thyroid transcription factor-1 （ITF-1） and napsin-A in the diferential diagnosis of poorly differentiated pulmonary non-small carcinomas between adenocarcinoma and squamous cell carcinoma. Methods 65 cases of poorly differentiated pulmonary non-small carcinomas were analysed, including 30 cases of adenocarcinoma and 35 cases of squamous cell carcinoma. The immunohistochemical technique was used to assay the expression of CK5/6, p63, TTF-1 and napsin-A in these patients. The sensi- tivity and specfieity of measurement of the four proteins in the diagnosis of lung squamous cell carcinoma and lung ad were also anal- ysed. Results CK5/6 and P63 were positive in 94.3% and 100% of adenocarcinoma and in 0% and 6.7% of squamous cell carcinoma, respective- ly. TTF-1 and napsin-A were positive in 96.7% and 100% of squamous cell carcinoma and in 2.9% and 0% of adenocarcinoma, respectively （all P〈 0.05）. The sensitivity and specificity of CK5/6 and p63 for the diagnosis of squamous cell carcinoma were 94.3%, 100% and 100%, 93.3%, re- spectively. The sensitivity and specificity of TTF-1 and napsin-A for the diagnosis of adenocarcinoma were 96.7%, 97.1% and 100%, 100%, re- spectively. Conclusion The combination of CK5/6, p63, TTF-1 and napsin-A was good immunohistochemical panel in differentiating adenocarci- noma from squamous cell carcinoma in poorly differentiated pulmonary non-small carcinomas.

关 键 词：非小细胞肺癌 鳞状细胞癌 腺癌 免疫组织化学 

分 类 号：R734[医药卫生—肿瘤]